Bulked Segregant Analysis Using the GoldenGate Assay to Locate the Rpp3 Locus that Confers Resistance to Soybean Rust in Soybean
Crop Sci Hyten et al.
49: 265
Supplemental Data
Files in this Data Supplement:
Supplemental Table 1
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Supplemental Table 1. Primers and positions on scaffold 60 of the sequence tagged sites (STS) used for haplotyping the Rpp3 region. Three SSR loci from the soybean consensus map along with their positions on scaffold 60 are also included. NS in the SNP column designates that no sequence was obtained from this primer pair due to multiple amplicons or low quality sequence.
Supplemental Table 2
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Supplemental Table 2. Genetic markers located on linkage group C2 between centimorgan (cM) position 96 cM and 139 cM on the soybean consensus map and the results for the bulked segregant analysis (BSA) using the GoldenGate assay. Sequence tagged site (STS) primer information and SNP flanking sequence are included for all SNP markers.
Supplemental Table 3
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Supplemental Table 3. SNP information for the 292 SNPs discovered in haplotyping the Rpp3 interval between Satt460 and Sat_263. Information includes the flanking seuquence around the SNP, consensus sequence obtained from the sequence tagged site (STS), and the SNP alleles for the soybean accessions that were haplotyped in this region.