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Figure 1. Alignment of the 395-bp fragment amplified for FAD2-1B cleaved amplified polymorphic sequence analysis in N98-4445A, N97-3363-3, PI423893, and ‘Brim’. The primers FAD2-1B_c_F and FAD2-1B_b_R designed for amplification are italicized. The restriction sites for HpyCH4III are underlined and the three single nucleotide polymorphisms (SNPs) are highlighted. The substitution from thymine to cytosine in one of the SNPs generates an additional restriction site for HpyCH4III endonuclease in PI423893.
