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Figure 2. Flowchart for large-scale selective genotyping and genetic mapping, including selection of phenotypic extremes from large-size segregating populations, phenotype confirmation, DNA extraction, genotyping, and marker–trait association analysis. (A) A procedure for most target traits which can be scored phenotypically for all individuals or fixed lines, and then high- and low-phenotypic extremes are selected for further analysis. (B) A procedure particularly suitable for abiotic and biotic stress tolerance where only the phenotypic extreme for tolerance is available under a target environment and comparison is made between the extreme and the phenotypic control that is randomly selected from the individuals or fixed lines under a normal environment.
