Click on image to view larger version.
Figure 2. (A) 2D pooling strategy. Rows and columns of primary plates condensed into individual wells in two adjacent columns of a 2D pool plate. (B) portion of a TILLING gel image (IRD800 channel) showing successive "row" and "column" pairs for two primary plates condensed to the same 2D pool plate, the first has no mutations and the second has two. Heavy boxes, bands corresponding to mutations; light boxes, position of bands for these mutations in the IRD700 channel. These mutations were then sequence verified.
