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Figure 1. PCR profiles showing strategy followed for deletion bin mapping: (a) Physical mapping of wEST–SSR PK123 (TC67549) on centromeric bin of 6DL (notice amplification in all the deletion stocks), Lanes M 100-bp ladder, 1 CS, 2 N6DT6A, 3 N6DT6B, 4 Dt6DS, 5 Dt6DL, 6 6DL-6, 7 6DL-1, 8 6DL-12, 9 6DL-11, 10 6DL-10. (b) Physical mapping of rEST–SSR SCM141 (BE495388) on interstitial bin defined by deletion breakpoints 5AL-10 and 5AL-17 (notice amplification in only some deletion stocks); Lanes M 100-bp ladder, 1 CS, 2 N5AT5B, 3 N5AT5D, 4 Dt5AL, 5 5AS-1, 6 5AS-3, 7 5AS-7, 8 5AS-10, 9 5AL-12, 10 5AL-10, 11 5AL-17, 12 5AL-23. (c) Physical mapping of wEST–SSR PK9 (BG274944) in distal bin of 2DS (notice absence of amplification in all the deletion stocks); Lanes M 100-bp ladder, 1 CS, 2 N2DT2A, 3 N2DT2B, 4 Dt2DS, 5 Dt2DL, 6 2DS-1, 7 2DS-5.
