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Figure 4. RT-PCR amplification for FAD2-1A_L, FAD2-1A_S, FAD2-1B_L, FAD2-1B_S, FAD2-2B, and FAD2-2C. Positive control reactions used tubulin56. Plus symbols indicate amplification of that transcript in a particular tissue, and minus symbols indicate no amplification. Tissue types are as follows: 6–10, developing pods 6–10 d after flowering (DAF); 13, developing pods 13 DAF; 17, developing pods 17 DAF; 19, developing pods 19 DAF; 21, developing pods 21 DAF; 26, developing pods 26 DAF; C3, cotyledons 3 days after emergence (DAE); R3, roots 3 DAE; FU, furled unifoliate 3 DAE; UU, unfurled unifoliate 4 DAE; C7, cotyledons 7 DAE; R8, roots 8 DAE; FT, furled trifoliolate 11 DAE; UT, unfurled trifoliolate 15 DAE; P, pods 76 DAE. All samples with a w were grown under the warm conditions and c for cool conditions. All samples with a -RT are controls with no reverse transcriptase for mRNA amplification, but still contain Taq DNA polymerase. The final sample in each set of reactions is a Williams 82 DNA based positive control.
