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Figure 3. Optimization of direct hybridization (DH) assay. SNP358 marker was genotyped across the F2:3 population from the cross of PI 96354 x Bossier using different length of probes and different hybridization temperature conditions. Data are presented as the log10 [(fraction of PI 96354 allele signal + 0.01)/(fraction of Bossier allele signal + 0.01)] versus total mean fluorescence intensity (MFI) of two alleles (Hirschhorn et al., 2000). Genotypes are represented by homozygotes for PI 96354 allele (
), heterozygotes (
), and homozygotes for Bossier allele (
) determined by the band sizes of the Satt358 SSR marker. (H.T. = hybridization temperature; ASO = allele-specific oligonucleotide).
