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Figure 5. Correlation between the F3'5'H 65-bp insertion and independently introduced w1 alleles. Polymerase chain reaction (PCR) fragments of 579 and 526 bp amplified from genomic DNAs isolated from multiple soybean lines (Table 1) with independently introduced W1 alleles were separated in a 3% NuSieve 3:1 agarose gel. The 53-bp longer PCR fragment was amplified from DNAs of cultivars and isolines with the w1 allele while the smaller 526 PCR fragment correlated with cultivars and lines with the W1 allele. Primers used were F35H-3980F and F35H-4558R. (A) Ethidium bromide stained gel showing the PCR fragments amplified from DNAs isolated from two independent plants of two isolines, L68-2056 and L66-14, which were used as parents for the segregating population shown in Table 2. (B) Ethidium bromide stained gel with PCR products amplified from DNAs isolated from 10 different soybean lines varying at the W1 locus (Table 1).
