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Figure 4. Alignment of three Glycine max F3'5'H cDNAs derived amino acid sequences. The three amino acid sequences were derived from (i) the cDNA annotated as F3'5'H in GenBank with accession number AY117551 and isolated from the cultivar Chin-Ren-Woo-Dou (W1) by R.-M. Liao, T.-M. Chu, and C.-S.Wang (http://www.ncbi.nlm.nih.gov); (ii) the cDNA sequence predicted from the L79-908 (W1) F3'5'H genomic sequence reported here; and (iii) the cDNA sequence predicted from the ‘Williams’ (w1) genomic sequence described here. The alignment was done with Multalin, a multiple sequence alignment on-line tool by Corpet (1988). The consensus sequence shows identical amino acids in the three sequences; divergences between two of the amino acid sequences are highlighted. The amino acid sequence derived from the Williams w1 recessive allele shows the amino acid substitutions introduced by the 65-bp insertion. This sequence terminates earlier (stop codon indicated by *) and is missing the last 44 amino acids. Overall, it is a predicted 42 amino acids shorter than the polypeptide derived from the wild-type sequence in the dominant W1 line. Underlined is the motif that distinguishes P450s from other heme-binding enzymes.
