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Figure 1. Amplification profiles of polymerase chain reaction (PCR)-based markers in the Dn2414 mapping population. (a) Bulked segregant analyses of four markers, Xgwm11, Xgwm18, Xrems1303, and Xiag95. Lanes 1 to 4 represent 2414-11, ‘Yuma’, resistant bulk, and susceptible bulk, respectively. Panels (b), (c), and (d) show the PCR patterns of Xgwm18, Xrems1303, and Xiag95, respectively. Lanes P1 and P2 represent 2414-11 and Yuma, respectively. The black or white arrows indicate the diagnostic band of specific markers. Lanes 1 to 23 represent 23 F2 individual plants. The genotypes of RWA resistance gene Dn2414 are shown for specific lanes/individuals: RR = homozygous resistant, Rr = heterozygous resistant, rr = homozygous susceptible.
