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Figure 8. Northern blot analysis of T2 plants derived from Line 28 that were exposed to 4 and 0°C for different periods of time: (A) accumulation of CBF3 transcripts in response to exposure to 4°C for 1, 3, 5, 7, 12, and 24 h (C is a wild-type plant and TN is a transgenic (T2) plant derived from Line 28 grown at 26°C); (B) T2 plants derived from Line 28 that were exposed to 0°C for 15 min, 30 min, and 1, 3, 6, 12, and 24 h (C is a wild-type plant); actin was used as a loading control.
