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Figure 2. Immunofluoresence confocal microscopy to confirm the localization of the hetrologous A. cellulolyticus endo-1,4-ß-endoglucanase E1 enzyme in transgenic maize leaf (left) compared with that of untransformed maize leaf (right). Both transgenic and the control leaves were treated with the E1 monoclonal primary antibody and the fluorescein isothiocyanate (FITC) anti-mouse secondary antibody. Green areas around cells of transgenic maize sample (left) indicate the apparent accumulation of E1 in apoplast. By permission of Current Opinion in Biotechnology (Sticklen, 2006).
