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Figure 3. Relationships between zoysiagrass freeze tolerance (LT50, the lethal temperature killing 50% of the plants) and (A) total reducing sugars in cold-acclimated plants (r2 = 0.41, P = 0.02), (B) glucose in cold-acclimated plants (r2 = 0.55, P = 0.004), (C) other reducing sugars in cold-acclimated plants (r2 = 0.29, P = 0.06), and (D) sucrose in cold-acclimated plants (r2 = 0.08, P = 0.34). Each point represents the mean of six replications. *, ** Significant at the 0.05 and 0.01 probability levels, respectively; NS, not significant.