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Figure 4. (A) Immunoblot of a 23-kDa dehydrin-like polypeptide from zoysiagrass genotypes with different freeze tolerances, showing a 23-kDa dehydrin probed with a 1:250 dilution of antidehydrin polyclonal primary antibody for cold-acclimated plants. Equal amounts of protein (25 µg) were loaded in each lane. (B) There was a relationship (r2 = 0.41, P = 0.018) between freeze tolerance (LT50, the lethal temperature killing 50% of the plants) and optical density (O.D.) of the 23-kDa dehydrin-like polypeptide band.
