Click on image to view larger version.
Figure 3. Immunoblots of buffer-soluble protein from zoysiagrass genotypes with different freeze tolerances. Immunoblots show dehydrin-like polypeptides probed with a 1:333 dilution of antidehydrin polyclonal primary antibody for nonacclimated (NA: Lanes 3, 5, 7, and 9) and cold-acclimated (CA: Lanes 4, 6, 8, and 10) zoysiagrass plants. Equal amounts of protein (25 µg) were loaded into each lane. Radish (Raphanus sativus) seed was used as a positive control with 10 µg of protein loaded in Lane 2. Dehydrin-like polypeptides and their predicted molecular masses are indicated (far right). Molecular weight (MW) markers represent proteins sized 104, 81, 48, 36, 27, and 19 kDa (Lane 1).
