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Figure 2. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles and immunoblots of buffer-soluble protein from zoysiagrass genotypes with different freeze tolerances. Gels show buffer-soluble protein profiles from nonacclimated (Lanes 2–14) and cold-acclimated (Lanes 15–27) zoysiagrass plants. Gels were loaded with equal amounts of buffer-soluble protein (25 µg) per lane. Gels were first stained with (A) Coomassie brilliant blue R-250 and then (B) Ag stained. Solid lines indicate predominant proteins present in nonacclimated and cold-acclimated plants and dashed lines indicated protein changes in response to cold acclimation with their predicted molecular masses (A and B, far right). (C) Immunoblots show 23- and 25-kDa dehydrin-like polypeptides probed with a 1:250 dilution of rabbit antidehydrin polyclonal primary antibody. ‘White icicle’ radish (Raphanus sativus) seed was used as a positive control with 3 µg of protein loaded in Lane 1. Solid lines indicate dehydrin-like polypeptides and their predicted molecular masses (C, far right). Molecular weight (MW) markers represent proteins sized 104, 81, 48, 36, 27, and 19 kDa (A, B, and C, far left).
