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a Dep. of Plant Biology and Pathology, Rutgers, The State Univ. of New Jersey, New Brunswick, NJ 08901-8520
b Dep. of Horticulture, Clemson Univ., Clemson, SC 29634-0319
c Dep. of Applied Economics and Statistics, Clemson Univ., Clemson, SC 29634-0319
* Corresponding author (haibol{at}clemson.edu).
| ABSTRACT |
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Abbreviations: PGR, plant growth regulator TE, trinexapac-ethyl USGA, United States Golf Association WAA, week after aerification WAIT, week after initial treatment
a Dep. of Plant Biology and Pathology, Rutgers, The State Univ. of New Jersey, New Brunswick, NJ 08901-8520
b Dep. of Horticulture, Clemson Univ., Clemson, SC 29634-0319
c Dep. of Applied Economics and Statistics, Clemson Univ., Clemson, SC 29634-0319
* Corresponding author (haibol{at}clemson.edu).
Dwarf bermudagrass (Cynodon dactylon x C. transvaalensis Burtt-Davy) and creeping bentgrass (Agrostis stolonifera L.) are planted for golf greens in the U.S. transition zone, but management regimes, such as the use of a plant growth regulator (PGR), often vary for long-term culture of the two species. The objective of this experiment was to investigate application regimens of the PGR trinexapac-ethyl (TE), on growth, quality, and performance of creeping bentgrass and bermudagrass putting greens in Clemson, SC. L-93 creeping bentgrass and TifEagle bermudagrass putting greens, established in summer 2002 and mowed at 3.2 mm, received TE over 12 wk in three regimens: 0.017 kg ha–1 wk–1, 0.033 kg ha–1 2 wk–1 (biweekly), or 0.05 kg ha–1 3 wk–1 (triweekly) from May to August 2003 and 2004. Creeping bentgrass was not discolored from TE, but unacceptable bermudagrass discoloration (>20%) occurred on one, two, and six dates for weekly, biweekly, and triweekly regimens, respectively. All TE regimens reduced bermudagrass clippings approximately 50% from nontreated bermudagrass across all dates, while creeping bentgrass clipping reductions, approximately 20 to 35% from nontreated grass, were inconsistent. The root masses of both species treated with TE regimens were similar to nontreated turf. Bermudagrass aerification recovery was reduced on two, two, and four dates after weekly, biweekly, and triweekly TE regimens, respectively. All TE regimens increased morning and evening bermudagrass golf ball roll distance approximately 25 cm from morning distances of nontreated turf.
Abbreviations: PGR, plant growth regulator TE, trinexapac-ethyl USGA, United States Golf Association WAA, week after aerification WAIT, week after initial treatment
| INTRODUCTION |
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Maintaining consistently uniform playing surfaces is an economically critical aspect of creeping bentgrass and bermudagrass golf green culture that requires intensive management (O'Brien, 1981; Foy, 1991; White, 1998; White et al., 2004; Hollingsworth et al., 2005). However, turf managers often compromise fundamental cultural practices to achieve desired putting green quality and ball roll distances. Practices that promote surface uniformity include lowering mowing heights, double cutting, and periodic rolling, which often reduce turfgrass aesthetics (Hartwiger et al., 2001; McCarty, 2005b). More important, maintaining putting green turf under conditions beyond their physiological capacities may deleteriously affect root growth, turf vigor, and recuperative capabilities from disease, traffic, heat, and other stresses (Beard, 1973; Hartwiger et al., 2001; Fagerness and Yelverton, 2001; Liu and Huang, 2002). Incorporating a plant growth regulator (PGR), trinexapac-ethyl (TE), into putting green maintenance is an alternative to these mechanical methods that may improve surface uniformity without negatively affecting plant health or aesthetics (Fagerness et al., 2000; Fagerness and Yelverton, 2001). Trinexapac-ethyl reduces leaf growth by inhibiting late gibberellin biosynthesis, thus often improving turfgrass quality and performance (Johnson, 1997; Rademacher, 2000; Ervin and Koski, 2001; Heckman et al., 2001a; Tan and Qian, 2003).
Reducing turfgrass shoot growth with TE effectively reduces mowing requirements, improves sod strength, and enhances turfgrass tolerance to shade, disease, and drought stress (Golembiewski and Dannerberger, 1998; Jiang and Fry, 1998; Qian and Engelke, 1999; Fagerness and Yelverton, 2000; Heckman et al., 2001b; Goss et al., 2002; Steinke and Stier, 2003; McCarty et al., 2004; Bunnell et al., 2005). Golf course superintendents also incorporate TE into golf green maintenance regimes to reduce ball roll friction caused by uneven shoot growth. In field experiments, Penncross creeping bentgrass treated with periodic TE applications showed temporary ball roll distance enhancements, but long-term improvements were more consistent when mowing height was reduced from 4.8 to 3.2 mm (Fagerness et al., 2000). In summer experiments where TE was applied at 0.04 kg ha–1 4 wk–1, L-93 creeping bentgrass ball roll distance was enhanced 1 to 2 wk after application, but reduced efficacy before sequential treatments prevented consistency of these effects (McCullough et al., 2006b).
Compared with creeping bentgrass, there has been limited long-term investigation of TE performance on dwarf-type bermudagrass. In a 2-yr experiment investigating nitrogen and TE use on TifEagle bermudagrass, TE applications at 0.05 kg ha–1 3 wk–1 enhanced ball roll distances 15% over nontreated turf, whereas ball roll distances were reduced with increased N rate and from morning to evening (McCullough et al., 2006a). Although TE did not mask diurnal differences in ball roll, bermudagrass treated with TE averaged 10% longer evening distances than morning distances of nontreated turf. In these experiments, spring applications of TE at 0.05 kg ha–1 caused significant discoloration but, TifEagle bermudagrass had better tolerance to sequential summer TE applications (McCullough et al., 2006c). These experiments suggest that TE has beneficial effects for bermudagrass golf green culture, but application regimens should be modified relative to creeping bentgrass greens.
Research is warranted to compare TE efficacy on dwarf bermudagrass with creeping bentgrass when grown as golf greens in the U.S. transition zone. Applying lower TE rates in weekly and biweekly regimens could provide more consistent creeping bentgrass growth inhibition than higher rates at longer (>2 wk) intervals. Furthermore, incremental TE applications at frequent intervals could reduce bermudagrass putting green discoloration without reducing total TE rate applied over the growing season. To test these hypotheses, we investigated the effects of TE application regimens on quality, growth, and performance of L-93 creeping bentgrass and TifEagle bermudagrass putting greens in a 2-yr field experiment.
| MATERIALS AND METHODS |
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Bermudagrass was aerified with 1.3-cm diameter hollow tines with 5 cm spacing and 10 cm lengths on 20 May and 28 July 2003 and 27 May and 28 July 2004. Cores were removed, and holes were filled as previously stated. Bermudagrass was initially fertilized in late April with 12 kg N ha–1 of ammonium nitrate plus 12 kg N ha–1 of an 18–3–18 (N–P–K) greens-grade granular fertilizer. In May bermudagrass was fertilized at 12 kg N ha–1 wk–1 with the 18–3–18 granular fertilizer until mid-August of both years.
Treatments and Experimental Design
Trinexapac-ethyl, in emulsifiable concentration (1EC), was applied at 0, 0.017 kg ha–1 wk–1, 0.033 kg ha–1 2 wk–1, or 0.05 kg ha–1 3 wk–1 with a CO2 sprayer calibrated at 700 L ha–1 beginning 1 May 2003 and 4 May 2004. Treatment application dates are presented in Table 1
. Experimental design was a randomized complete block with three replications of 1.5- x 2.1-m plots.
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Clippings were harvested weekly before scheduled TE applications on both the creeping bentgrass and bermudagrass greens, oven-dried at 80°C for 72 h, and then weighed. Two root samples measuring 600 cm3 (30 cm x 20 cm2) were taken per plot on 1 June, 29 June, and 26 July in 2003 and 31 May, 28 June, and 27 July in 2004. Root sa mple holes were backfilled with a similar sand medium on both greens. Roots were washed free of soil and organic matter over sieve screens that prevented loss of root fragments, oven-dried at 80°C for 72 h, and then weighed. Dry weight from the two samples was pooled for each plot before data analysis.
Weekly ball roll distances were measured on the bermudagrass green beginning 1 wk after initial treatment (WAIT). Sampling dates in 2003 were 8, 16, 24, 31 May; 6, 13, 21, 26 June; and 5, 11, 18, 25 July. In 2004, sampling dates were 12, 19, 26 May; 3, 11, 16, 23, 28 June; and 6, 13, 19, 26 July. Six ball roll measurements (three rolls in opposite directions) were made per plot with a 38-cm Stimpmeter (Gaussoin et al., 1995) in the morning from 9:00 to 11:00 a.m. and in the evening at >5:00 p.m. Morning ball roll measurements were made about 2 h after mowing. The Stimpmeter was raised until the golf ball rolled off the cleft located on the opposite end. Ball roll distances were obtained with tape measures laid parallel with plots, and the six rolls were pooled for data analysis. Ball roll distances obtained with the 38-cm Stimpmeter were converted to a standard USGA Stimpmeter (76-cm length) with methods described by Gaussoin et al. (1995).
Data Analysis
Data were subjected to analysis of variance. Clipping yield and seed-head cover were converted to percent of nontreated turf for results presentation with the equation
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| RESULTS |
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Ball Roll
Treatment interactions with year and date were not detected for bermudagrass ball roll; thus, results were pooled over all sampling dates. For bermudagrass treated with TE, a.m. and p.m. ball roll distances increased 20 to 28 cm longer than a.m. distances of nontreated bermudagrass (Table 2). Ball roll was reduced by 10 cm from a.m. to p.m. for nontreated bermudagrass, but diurnal differences were masked by all TE regimens.
Root Mass
Treatment interactions with year and sampling date were not detected for root mass. Both creeping bentgrass and bermudagrass treated with TE had root mass similar to nontreated turf on all sampling dates (data not shown). Creeping bentgrass root mass declined by 48 and 67% from May to July of 2003 and 2004, respectively (data not shown). Bermudagrass root mass declined 52% from May to July in 2003 but was similar across dates in 2004 (data not shown).
Aerification Recovery
Year-by-treatment interaction was detected for bermudagrass aerification recovery; thus, years are presented separately. All TE treatments reduced bermudagrass recovery by 20 to 40% 1 wk after aerification (WAA) in May 2003; however, recovery was similar to nontreated turf by 2 WAA (Fig. 3
). In 2004 the 0.05 kg ha–1 3 wk–1 TE regimen reduced aerfication recovery ?40% 1 and 2 WAA on 27 May. Bermudagrass receiving TE at 0.017 kg ha–1 wk–1 and 0.033 kg ha–1 2 wk–1 had similar aerification recovery to nontreated turf. Following the July 2003 aerification, turf recovery was reduced 20 to 30% by all TE regimens 1 WAA, but bermudagrass fully recovered by 2 WAA. Bermudagrass treated with TE had similar aerification recovery to nontreated turf in August 2004.
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| DISCUSSION |
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Creeping bentgrass discoloration from TE was never observed in this experiment, which is consistent with previous research (Fagerness et al., 2000; McCullough et al., 2006b). TifEagle bermudagrass was most sensitive to TE in May, but incremental weekly and biweekly applications helped mitigate turf discoloration from triweekly regimens without compromising total TE applied over 12 wk. Johnson (1994) noted similar leaf discoloration of Tifway bermudagrass following initial TE treatments of 0.2 kg ha–1 in late May, but turf generally recovered to acceptable levels within 1 to 2 wk. Fagerness and Yelverton (2000) also noted discoloration of Tifway bermudagrass from initial spring TE treatments at 0.11 kg ha–1, which was attributed to temperature in other experiments (Fagerness et al., 2002). Differences in tolerance to rates and interval of TE applications among species are likely due to growth and plant metabolism during late spring and early summer. Furthermore, triweekly applications of higher TE rates likely inhibited gibberellin synthesis to a greater extent following initial applications, which exacerbated dwarf bermudagrass discoloration from early summer applications (Pinthus et al., 1989). Results with TifEagle bermudagrass golf greens suggest applying triweekly TE regimens in weekly and biweekly increments at lower rates may effectively reduce these deleterious effects without reducing total TE rates applied.
While applying lower TE rates at more frequent application intervals effectively reduced bermudagrass discoloration, treatments resulted in similar clipping yield reduction, ball roll enhancement, and seed-head suppression compared with the triweekly regimen without restricting root growth. Clipping reductions from TE regimens resulted in ball roll distance enhancements in morning and evening that were greater than morning distances of nontreated turf over all dates. Results are consistent with other experiments on TifEagle bermudagrass with nitrogen and TE where evening distances of TE-treated turf were greater than morning distances of nontreated (McCullough et al., 2006a). In another study, creeping bentgrass ball roll distances were inconsistent over the 2 yr, but weekly, biweekly, and triweekly TE regimens enhanced ball roll on six, three, and four dates, respectively (McCullough et al., 2005).
Biweekly and triweekly TE applications on creeping bentgrass initially reduced clipping yield, but clippings were generally similar to nontreated turf on days of sequential applications. Weekly TE applications of 0.017 kg ha–1 did not initially or consistently reduce creeping bentgrass clipping yield, but fluctuation in clipping reductions appeared less substantial than other TE regimens. In other experiments, creeping bentgrass ball roll distances were infrequently enhanced, which was probably due to inconsistent clipping reductions (Fagerness et al., 2000; McCullough et al., 2006b). Results suggest that higher rates of TE could be applied more frequently on creeping bentgrass greens, such as 0.05 kg ha–1 biweekly, under moderate fertility regimes (6 kg N ha–1 wk–1) with spring and fall aerifications. However, the significance of TE effects could vary under different management regimes, such as routine topdressing, verticutting, or rolling, which warrants further investigation.
TifEagle bermudagrass produced greater seed heads in 2003 than 2004, which was likely the result of maturity and cooler spring temperatures in May 2003. Applications of TE reduced seed-head cover but did not completely suppress bermudagrass seed heads. Johnson (1994) noted that TE suppressed seed heads of higher-mowed bermudagrasses by >70%. In other experiments, TE provided partial seed-head suppression, but increased nitrogen fertility from 6 to 24 kg N ha–1 wk–1 reduced TifEagle bermudagrass seed-head coverage from 17 to 0% cover (McCullough et al., 2006c). Results suggest that TE and nitrogen fertility (>12 kg N ha–1 wk–1) during periods of seed-head formation would provide effective suppression on dwarf bermudagrass greens.
TifEagle bermudagrass recovery from aerifications was inhibited from TE applications. However, triweekly applications of TE at 0.05 kg ha–1 reduced aerfication recovery more frequently than weekly and biweekly regimens of lower rates. Inhibited aerification recovery from the triweekly TE regimen may have been exacerbated by bermudagrass foliar discoloration in both years. Conversely, incremental applications of weekly and biweekly TE treatments reduced discoloration and may have resulted in less-frequent recovery inhibition following aerifications. Previous studies noted that TifEagle bermudagrass lateral regrowth was inhibited by TE applications (Bunnell et al., 2005; McCullough et al., 2006c). These experiments also observed that increased nitrogen from 6 to 24 kg ha–1 wk–1 stimulated lateral growth, which improved recovery from aerifications. Results suggest that withholding TE before aerifications on bermudagrass golf greens while increased nitrogen fertility could enhance turf recovery. Since creeping bentgrass was aerified in spring and fall, effects of TE on aerification recovery were not measured during the test period, but further investigation is warranted.
Finally, conversion to improved species or cultivars may be desirable for putting greens in transition-zone golf courses. However, long-term TE use on bermudagrass greens will differ in rates and regimens from creeping bentgrass management. Managing TifEagle bermudagrass with TE in the transition zone may require lower rates than creeping bentgrass golf greens; creeping bentgrass managers could likely be more aggressive with TE use during spring and summer months. Further investigations are needed in the U.S. transition zone with other creeping bentgrass and dwarf bermudagrass cultivars. While these results have promising implications for TifEagle bermudagrass, further research should investigate effects of fertility, application timings, and mowing heights on responses of other dwarf bermudagrass cultivars, such as Champion and MiniVerde, to these TE regimens.
| NOTES |
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Received for publication April 21, 2006.
| REFERENCES |
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