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Figure 6. Comparative analysis of the NaCl–- and mannitol-induced expression of CBF1, GolS2, and GolS3 homologs in Solanum species: C = control, M = mannitol, S = NaCl. Gene expression analysis was performed after 2 d of exposure to a high concentration of NaCl (200 mmol/L) or mannitol (200 mmol/L) in hydroponics. Expression analysis was performed by semiquantitative real-time polymerase chain reaction. Gel images were representatives of the profiles from three independent biological replicates with two technical replicates each.