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Figure 1. Polyacrylamide gel electrophoresis showing the segregation pattern of the four microsatellite markers (A) Xgwm47, (B) Xgwm120, (C) Xbarc101, and (D) Xwmc175 in a subset of the F2 progenies from a cross between Sr9a near-isogenic lines (‘Chinese Spring’ and ISr9a-Ra); M is a 10-base pair (bp) ladder, 1 is resistant parent ISr9a-Ra, 2 is susceptible parent Chinese Spring, R is resistant F2 progeny, S is susceptible F2 progeny, and H is heterozygous resistant F2 progeny. Arrowheads indicate the size of the band associated with the Sr9a gene in coupling linkage (A–C) and also in repulsive linkage (D). Recombination or crossing over between the marker allele and the resistance allele is indicated by X.
