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Figure 2. Generation and evaluation of herbicide-resistant bahiagrass: (a) regeneration of plants from embryogenic bahiagrass callus following gene transfer; (b) transgenic bahiagrass plants established in soil in the greenhouse; (c) response of nontransgenic, wild-type bahiagrass (WT) and transgenic bahiagrass line 4 or (d) line 24 expressing the bar gene, 14 d after application of 0.2, 0.5, or 1.0% glufosinate ammonium under greenhouse conditions; (e) seed-derived progeny plants of transgenic bahiagrass; (f) transgenic bahiagrass lines expressing the bar gene (center) surrounded by nontransgenic bahiagrass plants before and (g) 14 d after application of 0.6% glufosinate ammonium under field conditions; (h) immunodetection of bar-encoded phosphinothricin acetyltransferase (PAT) in T0 transgenic lines or (i) seed-derived progeny of transgenic plants by lateral flow membrane assay (Libertylink Stix test). Protein extracts with detectable levels of PAT display a PAT-specific band indicated by the arrow, whereas extracts from nontransgenic, wild-type (WT) bahiagrass show a separate band, common to all lines, which indicates the functionality of the lateral flow membrane strip.
