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Figure 1. Transcript analyses for enhanced mitochondrial orf107 transcript processing. Total leaf RNAs were prepared, and RNA from 2.5 g tissue was blotted to membranes and probed with the orf107 clone pHC104, which spans orf107 and carries sequences of atp9 (Tang et al., 1996b). A. S05-37, #38; B. S05-38, #40; C. S05-38, #49; D. a male-fertile BC15F2 resulting from pollination of A3Tx398 with IS1112C; E. S05-36, #176; F. S05-37, #67; and G. S05-38, #48. The 1110-, 870-, and 810-nt transcripts represent whole-length orf107 transcripts, while the 380-nt transcript results from processing. The 650-nt transcript is derived from atp9.
