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Figure 2. Frequency distribution of the number of selected individuals in the F1 generation of the topcross (TCF1) (A) and doubled haploid lines (DHs) (B). The parents for the topcross were HM14BS, Sunstate, and Silverstar+tin. There were 500 individuals grown in the TCF1, 50 seeds were taken from each selected TCF1 individual to produce the F2 generation of the topcross (TCF2), and 50 DHs were produced from each selected TCF2 individual. In the TCF1 generation, homozygous selection was applied for Rht-B1a and Glu-B1i, and enrichment selection for Rht8, Cre1, and tin. In the TCF2 generation, homozygous selection was applied for Rht8, and enrichment selection for remaining target alleles. In DHs, only the target genotype was selected.
