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Fig. 1. Plasmid map of PV-GMBT01. Not I fragment contains the Bt. expression cassette. ori = microbial origin of replication. Either CP4 EPSPS (5-enolpyruvulshikimate-3-phosphate synthase [EPSPS] sequence isolated from Agrobacterium sp. strain CP4) or NPTII (neomycin phosphotransferase) was the selectable marker used in plant transformation. Spectinomycin or streptomycin resistance was used as a bacterial marker (aadA). Restriction enzyme sites for Eco RV and Not I are shown.