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Fig. 4. CAPS analysis of normal versus mutant GmKAS IIA genes from soybean. (A) Partial genomic sequence of GmKAS IIA and GmKAS IIB genes from C1727 showing exon (shaded) and intron (nonshaded) sequences in the vicinity of the DdeI site used for CAPS analysis. The locations of primer sequences used to specifically amplify the GmKAS IIA isoform are shown with arrows. An asterisk is located above the polymorphic A residue that creates a unique DdeI site in the GmKAS IIA gene from C1727 that is a G residue in the corresponding gene from Century. All DdeI recognition sites (CTNAG) within the displayed sequences are underlined. (B) DdeI digestion of genomic DNAs amplified from Century (Fap2, Fap2), C1727 (fap2, fap2), and N02–4441 (fap2, fap2) using the primer sequences shown in (A). Arrows point to the 220- and 131-bp fragments diagnostic of germplasm possessing the mutant GmKAS IIA gene. M, 50-bp molecular weight ladder.
