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Fig. 1. Comparison of the Wx exon 1-intron 1 junction sequences from rice cultivars with high (H) and intermediate (I) amylose content (AC). Exon and intron sequences are shown in capital and lowercase letters, respectively. The complete sequence is given only for cultivars with high AC, and only those that differ from the corresponding nucleotides are shown in boxes for cultivars with intermediate level of AC. The dashes (–) indicate nucleotide deletions in the intermediate AC cultivar, compared to the corresponding sequence of the high AC cultivar. The bolded sequences represent the recognition site by AccI in the high AC cultivar, where it is not due to the G to T mutation in the case of the intermediate AC cultivar. The primers used for the CAPS marker PCR-AccI determination are underlined.
