Click on image to view larger version.
Fig. 2. In situ probing with total genomic DNA in engineered chromosomes 1R. Probe signal is white; block signal is gray. Pairs from left to right: chromosomes 1R.1D5+10-2 and Valdy, probed with total genomic DNA of wheat with rye genomic DNA used as the block. Chromosomes 1RS.1DL and 1RS64:4.1DL probed with rye genomic DNA and wheat genomic DNA as the block. Two chromosomes MA1: the one on the left probed with wheat genomic DNA and rye genomic DNA as the block; the one on the right with rye genomic DNA and wheat DNA as the block. Probing with rye probe in 1RS64:4.1DL and MA1 shows a band of weaker signal in the middle of the satellite (single arrows). Probing with wheat probe in MA1 shows two distinct hybridization sites: the upper one delimits the 1BS insert with Gli-B1; the lower one the 1BS insert that eliminates the Sec-1 locus. Positions of signals relative to the telomere vary because of the presence/absence or size of the telomeric C-band from 1RS on some chromosomes.
