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Fig. 4. The probability of obtaining a consensus genotype (at least two correct microsatellite genotypes) for a given number of repeated genotypings (independent PCRs). The simulation was based on the observed error rate in an independently repeated genotyping experiment as described in this paper. The simulation result shows that 98.7% of the consensus genotype can be identified with a minimum of three PCR repetitions, and 99.7% of the consensus genotypes can be identified with four PCR repetitions. Further increase of PCR repetitions offers little further improvement in terms of genotyping accuracy.
