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Fig. 6. Comparative analysis of dehydration-induced DREB1 gene expression in PI 407155 (G. soja) and Essex (G. max). (A) GlyDREB1 transcripts were detected in PI 407155 by northern blot at 12 d after dehydration stress (+, with irrigation; –, without irrigation), but not in Essex with or without dehydration stress and well-watered PI 407155. The glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was used as control probe for sample loading. (B) Semi-quantitative PCR showing the differential GlyDREB1 temporal expression patterns between PI 407155 and Essex. The GlyDREB1 expression in PI 407155 exhibited a significant increase from the basal level beginning at 12 h after the dehydration stress. Expression in Essex remained a relatively constant (basal) level during the 36 h duration of dehydration stress. The constitutive expression of the actin gene is shown as negative control.
