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Fig. 2. Proteome analysis of two G. max P34-null accessions by 2-dimensional protein analysis. Proteins were separated using a pH gradient from 3.0 to 10.0 in the first dimension and 4 to 16% (w/v) gels in the second. Total proteins were detected by Coomassie blue stain [in each case, upper gel (i)] and a western blot performed on a replicate gel using P34-specific monoclonal antibody [in each case lower blot (ii)]. The cv. Kunitz (A) was used as a positive control and compared with the two P34-null accessions G. max PI603570A (B) and G. max PI567476 (C).
