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Fig. 1. One-dimensional protein profile and western blot analysis of P34-low expressing accessions. Total protein was extracted from mature seeds, separated on a 4 to 18% (w/v) gradient gel, stained with Coomassie blue [in each case upper figure (i)], and a replicate gel blotted for immuno-detection using the P34 specific monoclonal antibody. Control cv. Kunitz is compared with seven accessions of G. soja (A) three wild perennials, * IL = University of Illinois no. IL0827 (PI509462), IL 0612 (PI 446991), IL 0956 (PI not known) (B) and two G. max accessions (C).
