Click on image to view larger version.

Fig. 3. SDS-PAGE of seed proteins in the lower-yielding transgenic wheat lines (numbered lines) and their nontransgenic parent Bobwhite (C). The gel positions and names of the high molecular weight glutenin subunit (HMW-GS) native to Bobwhite are indicated to the left. Numbered lanes contain protein extracts from field-grown seeds from homozygous transgenic wheat lines T11 Hybrid-C (1), T7 Hybrid-B (2), T6 Dx5 + Dy10-E (3), T5 Dx5-L (4), T5 ShortDx5-F (5), T8 LongDx5-E short (6), and T5 Dx5 + Dy10-C (7). Arrowheads to the right of the individual lanes indicate expected locations of bands corresponding to transgene-encoded HMW-GS. Open circles to the right of individual lanes mark locations of bands of unexpected sizes and unknown identity.