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Fig. 2. SDS-PAGE of seed proteins in transgenic wheat lines (numbered lanes) and their nontransgenic parent Bobwhite (C). Arrowheads to the left of the individual lanes indicate expected locations of bands corresponding to transgene-encoded high molecular weight glutenin subunit (HMW-GS). The gel positions and names of the HMW-GS native to Bobwhite are indicated to the right. (A) Numbered lanes contain protein extracts from greenhouse-grown seeds from homozygous transgenic wheat lines expressing transgene-encoded native HMW-GS: T3 Ax2*-A (1), T3 Dy10-A (2), T6 Dy10-E (3), T5 Dx5-G (4), T5 Dx5-J (5), T7 Dx5+Dy10-A (6), T7 Dx5+Dy10-D (7), and T7 Dx5+Dy10-G (8). (B) Numbered lanes contain protein extracts from greenhouse-grown seeds from homozygous transgenic wheat lines expressing transgene-encoded recombinant HMW-GS: T11 Hybrid-C (1), T12 Hybrid-G (2), T6 LongDx5-C (3), T5 LongDx5-G (4), T4 ShortDx5-A (5), and T8 ShortDx5-D (6). (C) Numbered lanes contain protein extracts from greenhouse-grown—except Hybrid-B, which was field-grown—seeds from homozygous transgenic wheat lines exhibiting transgene-mediated cosuppression of HMW-GS: T9 Hybrid-A (1), T5 LongDx5-A (2), T4 Dx5-D (3), T5 Dx5-A (4), and T7 Hybrid-B (5). Lane BAR contains T11 seed extract from BAR-D.
