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Fig. 1. Diagrammatic representation of the high molecular weight glutenin subunit (HMW-GS) DNA sequences introduced by genetic transformation. Thickest boxes are coding regions, medium boxes are 5' flanking regions including promoters, and thin boxes are 3' flanking regions including transcription termination and polyA addition sites. Sequences from the native wheat gene Glu-D1–2 (Dy10) are in black, native gene GluD1–1 (Dx5) are in white, and native gene Glu-A1–1 (Ax2*) are in diagonal stripes. The coding sequences are divided into three regions corresponding to HMW-GS protein domains: the short N-terminal nonrepetitive region, the long repetitive region, and the short C-terminal nonrepetitive region shared by all HMW-GS. Shorthand names used in this report for the HMW-GS are to the left. (The pBluescript plasmid backbones are not shown.)
