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Fig. 4. Amplification of products using DNA extracted from seed samples from tall fescue and ryegrasses. DNA extraction was performed on 20-seed samples from the following grasses: tall fescue cv. Kentucky 31, up to 90% infected; Italian ryegrass cv. Jumbo, 34% infected; and perennial ryegrass cv. Express, 64% infected. Lanes: MW, Invitrogen 1 kb plus DNA ladder; (1) N. coenophialum, tall fescue; (2) E+ seed, tall fescue; (3) E– seed, tall fescue; (4) N. lolii, perennial ryegrass; (5) E+ seed, perennial ryegrass; (6) E– seed, perennial ryegrass; (7) E+ seed, Italian ryegrass; (8) E– seed, Italian ryegrass; (9) 0.5 ng of Claviceps pupurea DNA; (10) water control, MW, Invitrogen 1 kb plus DNA ladder. Reactions of seed samples used approximately 1 ng of isolated DNA, and pure fungal DNA controls utilized 0.1 ng (lanes 1 and 4).
