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Figure 2


Fig. 2. Determination of optimal temperature range for touchdown PCR reaction for detection of Neotyphodium sp. Numbers above lanes denote the temperature range (°C) used in the touchdown step. MW, Invitrogen 1 kb plus DNA ladder. (A) PCR reaction using 1 ng DNA from N. coenophialum fungus isolated from tall fescue. (B) PCR reaction using 1 ng DNA isolated from 20 N. coenophialum E+ seeds (tall fescue cv. Kentucky 31, 90% infected).





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The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
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Environmental Quality		 The Plant Genome