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Fig. 1. Alignment of Neotyphodium spp. tub2 sequences isolated from infected seed from tall fescue and ryegrass species. Sequence designation: TUB2-Fa, Festuca arundinacea (Genbank accession no. AY865627); TUB2-Lm, Lolium multiflorum (Genbank accession no. AY865629); TUB2-Lp, Lolium perenne (Genbank accession no. AY865628); and TUB2-Cp-Claviceps purpurea (Genbank accession no. AF062646). CLUSTAL X sequence alignment is in 5'–3' orientation. Primer names and their sequences are located above the tub2 sequence alignment at their annealing sites (see boxes). Arrows denote direction of amplification. Asterisks denote conservation among all tub2 sequences shown.
