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REGISTRATIONS OF GENETIC STOCKS

Registration of Rusty Durum Wheat

USDA-ARS, Northern Crop Science Laboratory, P.O. Box 5677, Fargo, ND 58105

^* Corresponding author (klindwod{at}fargo.ars.usda.gov)

Rusty (Reg. no. GS-155, PI 639869), a genetic stock of durum wheat (Triticum turgidum L. var. durum) that is a near universally susceptible to wheat stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & Henn.) was selected and released in December 2004 by the USDA-ARS Northern Crops Science Laboratory, Fargo, ND, and North Dakota State University. Rusty was selected from a euploid BC₁F₃ plant having the parentage Langdon 4D(4B)/Line 47–1//Line 47–1. Langdon 4D(4B) is a disomic substitution line in which chromosome 4B of the durum wheat ‘Langdon’ (CItr13165) was substituted by chromosome 4D from the hexaploid wheat (T. aestivum L.) Chinese Spring (CItr14108) (Joppa and Williams, 1988). The parentage of Line 47–1 involves PI 192334 and either Langdon or a Langdon aneuploid (L.R. Joppa, personal communication, 1997). PI192334 is a selection known as Marruecos 9623 (Weeraratne and Williams, 1971), Marrocos 9623 (Williams and Gough, 1968), Maruccos 623 (USDA-ARS Cereal Disease Laboratory, 2004), or simply Marrocos (USDA-ARS National Genetic Resources Program, 2004). PI 192334 is moderately susceptible to stem rust, but it carries a single thermosensitive gene, temporarily designated SrM (USDA-ARS Cereal Disease Laboratory, 2004), that confers an intermediate infection type (IT) of 13 or 31 (Stakman et al., 1962) to pathotype, Pgt-LBBL, at low temperature (19–21°C), but an IT of 34 at temperatures above 21°C. Line 47–1 also carries this gene.

The pathotype Pgt-LBBL is an important pathotype for screening germplasm to identify new stem rust resistance genes since it is avirulent to most host resistance genes. When Pgt-LBBL is used to identify resistant progenies in crosses involving either Marruecos 9623 or Line 47–1, the presence of the SrM gene interferes with selection of resistant progenies and causes susceptible progenies to be misclassified as resistant. Selection of a durum genotype having temperature-insensitive susceptibility to Pgt-LBBL, indicating that the SrM gene has been eliminated, would increase the utility of Pgt-LBBL for selection or genetic analysis of new stem rust resistance genes.

To initiate the selection of Rusty, 13 BC₁F₁ plants that had a susceptible reaction to pathotypes Pgt-JCMN and Pgt-LBBL were selected. The pathotype Pgt-JCMN had been used in the prior study because it was a gray-brown color mutant which allowed for simultaneous testing of two pathotypes of stem rust on a single leaf. We screened for temperature-insensitive susceptibility to Pgt-LBBL in BC₁F₂ families, each derived from one of the 13 BC₁F₁ selected plants using procedures previously described (Williams et al., 1992) except that greenhouse temperatures did not exceed 20°C. Plants were scored for IT, and two families were found to be homogeneous for temperature-insensitive susceptibility to Pgt-LBBL. An additional 25 plants per BC1F3 family were screened for temperature-insensitive susceptibility to Pgt-LBBL and also selected for plant fertility and morphological characteristics. A single BC₁F₃ plant, originally tested as 47–1R1, was selected and named Rusty.

Rusty resembles Langdon in plant type with the exception that Rusty has black chaff conferred by the Bg gene on chromosome 1A. Following its selection, Rusty was included in additional trials to test thermosensitivity to Pgt-LBBL. In those trials, plants were inoculated and grown under low (19–21°C) and high (21–28°C) temperatures. Rusty consistently had a susceptible reaction (IT 34) at both temperatures. In addition to trials with Pgt-LBBL, Rusty has been tested in the greenhouse for stem rust reaction to pathotypes Pgt-TPMK, -THTS, -TMLK, -TCMJ, -RTQQ, -MCCF, -QTHJ, -QCCJ, -HKHJ, and -JCMN, and was found to have a susceptible reaction (IT 34 or 4) to each pathotype.

Rusty will be useful to researchers studying genetics of stem rust resistance in tetraploid wheats or in crosses designed to select monogenic progeny from a parent containing multiple genes for stem rust resistance. Small quantities (5–10 g) of seed will be available at the USDA-ARS, Cereal Crops Research Unit, Fargo, ND 58105. Plant variety protection will not be sought. It is requested that this registration article be cited and appropriate recognition of the USDA-ARS Cereal Crops Research Unit be given if Rusty is used in published research or if it contributes to the development of a cultivar.

NOTES

Registration by CSSA.

Accepted for publication August 31, 2005.

REFERENCES

• Joppa, L.R., and N.D. Williams. 1988. Langdon durum disomic substitution lines and aneuploid analysis in tetraploid wheat. Genome 30:222–228.
• Stakman, E.C., D.M. Stewart, and W.Q. Loegering. 1962. Identification of physiologic races of Puccinia graminis var. tritici. USDA-ARS E617. Rev. ed. Scientific Journal Series Paper no. 4691. Minnesota Agric. Exp. Stn., St. Paul, MN.
• USDA-ARS Cereal Disease Laboratory. 2004. Wheat stem rust resistance genes: Source, gene location, low infection type(s) and tester lines [Online]. Available at www.cdl.umn.edu/res_gene/wsr.html (verified 28 Nov. 2005). Cereal Disease Lab., St. Paul, MN.
• USDA-ARS National Genetic Resources Program. 2004. National Plant Germplasm System- NPGS [Online]. PI 192334. Available at http://www.ars-grin.gov/cgi-bin/npgs/html/acchtml.pl?1163805 (verified 28 Nov. 2005). Natl. Germplasm Resources Lab., Beltsville, MD.
• Weeraratne, H., and N.D. Williams. 1971. Inheritance of resistance to stem rust in six durum wheats. Crop Sci. 11:915–918.[Abstract/Free Full Text]
• Williams, N.D., and F.J. Gough. 1968. Inheritance of stem rust resistance of tetraploid wheats. p. 239–234. In Finlay, K.W., and K.W. Shepherd ed. Proc. Int. Wheat Genet. Symp., 3rd. Canberra, Australia. 5–9 Aug. 1968. Aust. Acad. Sci., Canberra.
• Williams, N.D., J.D. Miller, and D.L. Klindworth. 1992. Induced mutations of a genetic suppressor of resistance to wheat stem rust. Crop Sci. 32:612–616.[Abstract/Free Full Text]

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