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a Dep. of Agron. and Soils, Univ. of Puerto Rico, P.O. Box 9030, Mayaguez, PR 00681
b Dep. of Horticulture, Univ. of Puerto Rico, P.O. Box 9030, Mayaguez, PR 00681
c Dep. of Plant, Soil and Entomological Sciences, Univ. of Idaho, Moscow, ID 83844
d USDA-ARS, IAREC, 24106 N. Bunn Road, Prosser, WA 99350
* Corresponding author (jbeaver{at}uprm.edu)
Bean golden yellow mosaic virus (BGYMV) resistant common bean (Phaseolus vulgaris L.) germplasm lines PR9771-3-2 (Reg. no. GP-239, PI 636494), PR0247-49 (Reg. no. GP-240, PI 636495), and PR0157-4-1 (Reg. no. GP-241, PI 636496) were developed and released collaboratively by the Puerto Rico Agricultural Experiment Station and the USDA-ARS, Tropical Agriculture Research Station (TARS). In the Caribbean Basin, BGYMV, a whitefly [Bemisia tabaci (Gennadius)] transmitted Begomovirus, can cause significant yield losses in beans (Gálvez and Morales, 1989). Pyramiding genes from the Durango and Mesoamerica bean races has led to the development of breeding lines with high levels of resistance to leaf chlorosis and pod deformation caused by BGYMV (Singh et al., 2000). The genetic base for BGYMV resistance, however, remains narrow. The emergence of BGYMV strains capable of overcoming the resistance conferred by the recessive resistance gene bgm-1 could pose a threat to bean production in Central America and the Caribbean.
G35172, a scarlet runner bean (P. coccineus L.) accession, was identified by CIAT scientists as a source of resistance to BGYMV (Beebe and Pastor-Corrales, 1991). Preliminary results suggested that the BGYMV resistance of G35127 was conferred by two genes (Muñoz-Perea, 2002). The genes were subsequently identified as a recessive gene for resistance to leaf chlorosis and a dominant gene for resistance to pod deformation (Osorno et al., 2003). Dr. Phil Miklas made the original interspecific cross with G35172 in Puerto Rico in 1992 at TARS. The small red breeding line PR9771-3-2 was derived from a backcross (HP8437-95/G35172//HP8437-95) made at TARS in 1993. HP8437-95 is a BGYMV susceptible small red breeding line from the cross 15R-148/3M-81. The BC1F2:3:5:7 lines were single plants selected in the field at the Isabela Substation for BGYMV resistance in leaves and pods in the F2, F3, and F5 generations. Results from screening with the SR2 Sequence Characterized Amplified Region (SCAR) marker (Urrea et al., 1996), suggested that PR9771-3-2, PR0247-49, and PR0157-4-1 do not have the bgm-1 gene for BGYMV resistance. The P. coccineus parent G35172 and the three germplasm lines, however, do have the SCAR marker SW-12 associated with a quantitative trait locus for resistance to BGYMV (Miklas et al., 2000). PR9771-3-2 has the SW13 SCAR linked to the I gene for BCMV resistance (Melotto et al., 1996). PR9771-3-2 has white flowers and a dark red seed color. PR9771-3-2 has a 100-seed weight of 22 g and an indeterminate growth habit (Type III). In Puerto Rico, PR9771-3-2 flowers approximately 35 d after planting (DAP) and matures 76 DAP.
PR0247-49, a shiny small black bean breeding line, was derived from the cross Morales/PR9771-1-3 made at the Finca Alzamora of the Mayaguez Campus of the University of Puerto Rico in February 1998. PR9771-1-3 is a BGYMV resistant breeding line from the cross HP8437-95/G35172//HP8437-95. The F1 generation was planted at the Isabela Substation in November 1998. Individual plants were selected from an F2 nursery planted at Isabela in November 1999. A total of 200 F2:3 lines were screened for BGYMV resistance in a nursery planted at Mayaguez in January 2000. F3 lines that expressed a uniform and high level of resistance to BGYMV, both in the leaves and pods, were bulked. The F3:4 generation was planted at the Isabela Substation in October 2000. The BGYMV resistance of PR0247-49 was confirmed in a F3:5 nursery planted at Isabela in June 2001. PR0247-49 does not have the SW13 SCAR for the I gene for BCMV resistance. PR0247-49 has purple flowers, a 100-seed weight of 20 g, a Type III growth habit, and flowers approximately 38 DAP and matures 74 DAP.
PR0157-4-1, a small white bean breeding line, was derived from the cross Arroyo. Loro/PR9771-1-3 was made at Mayaguez in February 1998. The F1 generation was planted at Isabela in November 1998, and subsequent generation advance, BGYMV testing, and development of PR0157-4-1 was the same as for PR0247-49. PR0157-4-1 has the SW13 SCAR linked to the I gene for BCMV resistance. In Puerto Rico, PR0157-4-1 has white flowers, a 100-seed weight of 23 g, and a Type III growth habit. In Puerto Rico, PR0157-4-1 flowers approximately 35 DAP and matures 76 DAP.
In Puerto Rico, PR9771-3-2, PR0247-49, and PR0157-4-1 are susceptible to common bacterial blight [caused by Xanthomonas campestris pv. phaseoli (Smith) Dye] and resistant to endemic races of rust [caused by Uromyces appendiculatus (Pers.) Unger]. The seed yield and agronomic traits of all three lines are similar to adapted Mesoamerican bean cultivars.
Limited quantities of seed for research are available from the corresponding author. We ask that appropriate recognition of source be given when this germplasm contributes to new cultivars or breeding lines.
NOTES
Accepted for publication March 31, 2005.
REFERENCES
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