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Fig. 2. Thermostability profiles of the ‘Morex’ and ‘Steptoe’ wild-type recombinant ß-amylase2 enzymes and the mutant enzymes made to assess the impact of single nucleotide polymorphisms on the thermostability. Enzymes were incubated for 10 min at temperatures from 0 to 70°C, and remaining activity was determined at 40°C. Means and standard deviations of activities from four to eight separate colonies are shown for each temperature. (A) Morex values are shown as squares and Steptoe values are shown as triangles. (B) Activities of the D238G are shown as open circles. M337T activities are shown as open triangles and the Q362R activities are shown as open squares. The Morex and Steptoe wild-type rBmy2 enzymes' thermostability profiles are shown for comparison. (C) Activities of the D238G/M337T double mutant rBmy2 are shown as open circles. Activities of the M337T/Q362R double mutant are shown as open squares. Activities of the D238G/Q362R double mutant are shown as open triangles. Morex and Steptoe wild-type rBmy2 profiles are shown for comparison. (D) Activities of the D238G/M337T/Q362R triple mutant are shown as open circles. Morex and Steptoe wild-type rBmy2 enzyme thermostability profiles are shown for comparison.
