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Published online 24 June 2005
Published in Crop Sci 45:1675-1678 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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REGISTRATIONS OF GENETIC STOCKS

Registration of N30-N56, N741, N743, N745, N747, U362, U363, U367, U369-U374, U389-U394, U396-U398, and U500 Sweetclover Genetic Stocks

K.P. Vogel*, H.J. Gorz and F.A. Haskins

USDA-ARS and Department of Agronomy and Horticulture, 344 Keim Hall, P.O. Box 830937, Univ.of Nebraska, Lincoln, NE 68583-0937

* Corresponding author (kpv{at}unlserve.unl.edu)

Forty-nine white-flowered sweetclover (Melilotus alba Medik.) genetic stocks [N30-N45 (Reg. GS-1–16, PI 549120–549135); N46-N53 (Reg. GS-17–24, PI 557503–PI 557510); N54-N55 (Reg. GS-25–Reg. GS-26, PI 629289–PI 629290); N741, N743, N745, N747 (Reg. GS-27–GS-30, PI 557511–PI 557514); U362, U363, U367 (Reg. GS-31, Reg. GS-32, Reg. GS-33, PI 557515–PI 557517); U369-U374 (Reg. GS-34–GS 39, PI 557518–PI 557523); U389-U394 (Reg. GS-40–GS 45, PI 557524–PI 557529); U396-U398 (Reg. GS-46–GS 48, PI 557530–PI 557532); U500 (Reg. GS-49, PI 557533)] (Table 1); and N56 (Reg. no. GS-50, PI 634019), a yellow-flowered sweetclover [Melilotus officinalis (L.) Lam.] genetic stock, were developed jointly by USDA-ARS and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln and were jointly released in May 2004. The genetic stocks, which contain unique combinations of genes and traits, were developed over more than three decades of cooperative sweetclover genetic research.


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  		Table 1. Phenotypes and genotypes of 49 Melilotus alba genetic stocks.

 
The 49 M. alba genetic stocks include a set of 16 lines, N30 through N45, which represent all possible homozygous combinations of four allelic pairs, Y/y, C/c, Cu/cu, and B/b (Table 1). The Y/y alleles affect seed color, and the C/c alleles are concerned with both seed and seedling color (Gorz et al., 1975). The Cu/cu and B/b genes affect coumarin (more accurately, o-hydroxycinnamic acid ß-D-glucoside) content (Gorz and Haskins, 1969) and ß-glucosidase activity (Haskins and Gorz, 1965), respectively. The development of these 16 lines involved both annual and biennial forms of M. alba, and the greenhouse conditions under which seed of these lines were produced did not permit distinguishing between these forms. Both forms may be present in these lines.

Lines N46 through N49, and N741, N743, N745, and N747, are two sets of four lines, each set representing all possible homozygous combinations of the Cu/cu (coumarin content) and B/b (ß-glucosidase activity) alleles. N46 through N49 are annuals. They were derived from an initial cross of cucubb biennial plants x CuCuBB plants of PI 165554, a small, annual, autogamous introduction from India, followed by six successive backcrosses of cucubb segregates to the CuCuBB annual parent. N741, N743, N745, and N747 are biennial lines. They are F21 generation lines derived from an initial cucuBB x CuCubb cross followed by self-pollination of a single doubly heterozygous plant in each generation from F1 to F17. The four homozygous genotypes were isolated in F18.

N50 through N53 are biennial lines representing all possible homozygous combinations of the Y/y and C/c allelic pairs. As indicated above, the C/c genes influence seedling color, and both Y/y and C/c affect seed color. These four lines are the F6 generation from a single F1 plant that was obtained from a cross of the N1 strain (yyCC genotype) x a line designated JF-1 (YYcc genotype). N54 and N55 are biennial lines that are homozygous for susceptibility and resistance, respectively, to stem canker (gooseneck) disease [caused by Ascochyta caulicola (Laub.)].

U389 is an annual line that was derived from a single plant of the introduction, PI 165554, mentioned above. All of the other U-numbered lines were developed following treatment of U389 seed with ethyl methanesulfonate. Although not always identified as such, U389 was the "wild-type" (+/+) line used in the referenced studies involving the lines that resulted from ethyl methanesulfonate treatment.

The normal parent (U389) and the chlorophyll-deficient mutants (U369, U371, U372, U373, U374, U396, U397, U398) were used by Markwell and coworkers (Bevins et al., 1993, 1992; Markwell and Chelgen, 1988; Markwell et al., 1986, 1985a, 1985b; Yang et al., 1990) and Nakitani and Baliga (1985) in their biochemical research. U389 also was used by Kneen and LaRue (1988) to create a series of non-nodulating mutants for studying the process of nitrogen fixation in legumes.

N56, a biennial strain of M. officinalis, was developed by crossing N27 (a large-seeded, high-coumarin, early-maturing M. officinalis) to N1 (a finestem, small-seeded, low-coumarin, late-maturing M. alba) with one backcross of finestem, low-coumarin F2 segregates to N27 followed by a second backcross to N29 (a low-coumarin strain of M. officinalis). N56 combines finestem growth habit and low coumarin content of M. alba with the large-seeded trait and early maturity of M. officinalis.

Registration of these genetic stocks supplements three previously released and registered biennial, yellow-flowered, sweetclover germplasms, N27, N28, and N29 (Gorz et al., 1992a, 1992b). Seed of all lines has been deposited in the National Plant Germplasm System. Requests for any of the 49 M. alba lines and N56 M. officinalis should be to the National Plant Germplasm System (http://www.ars-grin.gov/npgs.orders.html; verified 9 March 2005). Seed should be scarified before planting. It is requested that appropriate recognition be made if these genetic stocks contribute to research or the development of a new breeding line or cultivar.

NOTES

Joint contribution of the USDA-ARS and University of Nebraska-Lincoln. Journal Series Paper No. 14852. Registration by CSSA.

Accepted for publication February 28, 2005.

REFERENCES





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