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REGISTRATIONS OF GERMPLASMS

Registration of LDX01-1-65 Soybean Germplasm with Soybean Cyst Nematode Resistance Derived from Glycine soja

^a Dep. of Crop Sciences, Univ. of Illinois, Urbana, 61801
^b USDA-ARS, 605 Airways Blvd., West Tennessee Exp. Stn., Jackson, TN 38301
^c Dep. of Agronomy, Iowa State Univ., Ames, IA 50011
^d Horticultural Sciences Dep., Univ. of Florida, Gainesville, FL 32611
^e USDA-ARS, Dep. of Agronomy, Iowa State Univ., Ames, IA 50011
^f Dep. of Crop and Soil Sciences, Michigan State Univ., E. Lansing, MI 48824

^* Corresponding author (bdiers{at}uiuc.edu)

LDX01-1-65 soybean [Glycine max (L.) Merr.] germplasm (Reg. no. GP-310, PI 636464) is a late maturity group III line that was developed cooperatively by the Illinois Agricultural Experiment Station at the University of Illinois, the Iowa Agriculture and Home Economics Experiment Station at Iowa State University, and the USDA-ARS. It was released in March 2004 because it has two quantitative trait loci (QTL) providing soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) resistance from the Glycine soja Sieb. and Zucc. (PI 468916). These G. soja resistance QTL may be useful in broadening the repertoire of SCN resistance genes in commercially produced soybean since they do not map to regions where SCN resistance genes in current cultivars are located.

LDX01-1-65 is a F₃ plant selection from a population developed through four backcrosses (BC4) with PI 468916 as the donor parent and the Iowa State University experimental line A81-356022 as the recurrent parent. PI 468916 is a G. soja plant introduction that was collected in 1982 from Liaoning Province, People's Republic of China (Bernard et al., 1989). A81-356022 is a selection from a cross between ‘Century’ x A76-304020, and A76-304020 is a selection from the crosses (‘Beeson’ x AP68-1016) x (L15 x ‘Calland’) (Wilcox et al., 1980; Probst et al., 1969a, 1969b). The parentage of AP68-1016 is ‘Clark’(5) x PI 84946-2, and the parentage of L15 is ‘Wayne’ x ‘Clark 63’ (Williams and Bernard, 1964; Bernard, 1966).

The two SCN resistance QTL from PI 468916 were mapped to linkage groups E and G in a population of F₂–derived lines from a cross between A81-356022 and PI 468916. After the first backcross, these QTL were confirmed in a BC₁F₂ population (Wang et al., 2001). Three additional backcrosses were made, and linked markers were used to select for the QTL during each generation of backcrossing. A BC₄F₁ plant heterozygous for resistance alleles at both QTL was self-pollinated and a population of BC₄F₃–derived lines were developed through single-seed descent. The lines were tested for SCN resistance in a greenhouse at the University of Illinois with PA3, a HG type 0 isolate (Race 3) of SCN (Niblack et al., 2002). This test resulted in a second confirmation of the SCN resistance QTL. The BC₄F₃–derived lines also were tested for agronomic traits in three Illinois environments during 2003 and two in 2004. The field locations were sampled for the presence of SCN and the end of season egg concentrations were estimated for each replication of the field tests.

LDX01-1-65 was selected for release because it was the highest yielding BC₄F₃–derived line in the population that was homozygous for resistance alleles at both QTL. The agronomic appearance of LDX01-1-65 is similar to a normal soybean line and it had no increased shattering compared with the recurrent parent. Across environments, LDX01-1-65 had 195 kg ha^–1 greater seed yield, 1.1 unit greater lodging (on a 1-to-5 scale), was 1 cm taller, and 3 d later in maturity than A81-356022, the recurrent parent. Compared with ‘Dwight’, a cultivar with SCN resistance from PI 88788 (Nickell et al., 1998), LDX01-1-65 yielded 197 kg ha^–1 less, was 8 d later in maturity, 27 cm taller, and had 1.8 units greater lodging. There was moderate SCN pressure in the field locations, with environments averaging from 86 to 4280 eggs 100 mL^–1 of soil at the end of the season. Although yield loss thresholds are difficult to estimate, it is likely that the SCN pressure in some environments were sufficient to cause the resistance genes to give a yield advantage (Brucker et al., 2005).

When inoculated with the SCN isolate PA3, the female index of LDX01-1-65 was 14, compared with 3 for PI 88788, 2 for the Dwight, and 95 for A81-356022. LDX01-1-65 also was inoculated in a greenhouse at the West Tennessee Experiment Station with PA2, a HG type 1.2.5.7 SCN isolate (Race 2), and PA5, a HG type 2.5.7 SCN isolate (Race 5). In the PA2 test, LDX01-1-65 had a female index of 57, compared with PI 88788 with 23. In the PA5 test, LDX01-1-65 had a female index of 3, compared with 53 for PI 88788. LDX01-1-65 is an indeterminate line with purple flowers, tawny pubescence, brown pod color at maturity, and yellow seeds with black hila.

Seed of LDX01-1-65 will be maintained by the Illinois Agricultural Experiment Station at the University of Illinois, Urbana, IL 61801. A small sample of LDX01-1-65 may be obtained from the senior author for research purposes, including the development and commercialization of new cultivars, for at least 5 yr. It is requested that appropriate recognition be made if LDX01-1-65 contributes to the development of new cultivars, germplasm, parental lines, or genetic stocks. No application will be made for U.S. Plant Variety Protection for LDX01-1-65.

NOTES

Contribution from the Illinois Agric. Exp. Stn., Urbana. Research supported in part by the Illinois Soybean Checkoff Board. Registration by CSSA.

Accepted for publication January 31, 2005.

REFERENCES

• Bernard, R.L. 1966. Registration of Wayne soybeans. Crop Sci. 6:305.
• Bernard, R.L., G.A. Juvik, and R.L. Nelson. 1989. USDA soybean germplasm collection inventory, Vol. 2(30). University of Illinois at Urbana-Champaign.
• Brucker, E., T. Niblack, F.J. Kopisch-Obuch, and B. Diers. 2005. The effect of rhg1 on reproduction of Heterodera glycines in the field and greenhouse and associated effects on agronomic traits. Crop Sci. 45(5).
• Niblack, T.L., P.R. Arelli, G.R. Noel, C.H. Opperman, J.H. Orf, D.P. Schmitt, J.G. Shannon, and G.L. Tylka. 2002. A revised classification scheme for genetically diverse populations of Heterodera glycines. J. Nematol. 34:279–288.[ISI]
• Nickell, C.D., G.R. Noel, T.R. Cary, and D.J. Thomas. 1998. Registration of ‘Dwight’ soybean. Crop Sci. 38:1398.[Free Full Text]
• Probst, A.H., F.A. Laviolette, K.L. Athow, and J.R. Wilcox. 1969a. Registration of Calland soybean. Crop Sci. 9:524.[Free Full Text]
• Probst, A.H., F.A. Laviolette, K.L. Athow, and J.R. Wilcox. 1969b. Registration of Beeson soybean. Crop Sci. 9:523–524.[Free Full Text]
• Wang, D., P.R. Arelli, R.C. Shoemaker, and B.W. Diers. 2001. Loci underlying resistance to Race 3 of soybean cyst nematode in Glycine soja plant introduction 468916. Theor. Appl. Genet. 103:561–566.[CrossRef][ISI]
• Wilcox, J.R., K.L. Athow, T.S. Abney, F.A. Laviolette, and T.L. Richards. 1980. Registration of Century soybean. Crop Sci. 20:415.[Free Full Text]
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