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Fig. 1. Effects of chilling treatments and age of flowers on seed coat pigmentation in Harosoy and its near-isogenic soybean lines, Harosoy-E1, Harosoy-e3e4, and Harosoy-E1e3e4. Pigmentation index (0, not pigmented to 4, severely pigmented), bars indicate standard error of the mean, numbers at each data point represent the number of seeds obtained from flowers exposed to chilling at a similar stage.