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Fig. 3. Southern blot analysis for nptII and cry3Bb1 cassette and coding region intactness and plasmid PV-ZMIR13 backbone. Ten micrograms of MON846 DNA (A1xA634) (lane 1) and MON863 DNA (Lanes 2 and 5) were digested with HindIII. Plasmid PV-ZMIR13 DNA (Lanes 3 and 4) was spiked into 10 µg of MON846 DNA and digested with HindIII. The membranes were probed with 32P-labeled full-length nptII coding sequence (Panel A), full-length cry3Bb coding sequence (Panel B), or two backbone probes encompassing the entire backbone sequence except for the nptII coding region (Panel C). High molecular weight DNA ladder (Gibco BRL, Gaithersburg, MD) was loaded on the long run and molecular weight markers II and IX (Roche, Indianapolis, IN) were loaded on the short run in all three panels. The arrows denote the sizes obtained from the molecular weight markers on the ethidium-stained gel.
