Crop Science Journal of Natural Resources and Life Sciences Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Published online 6 May 2005
Published in Crop Sci 45:1084-1091 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Abstract Freely available
Right arrow Figures Only
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Related articles in Crop Science
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fu, Y.-B.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Fu, Y.-B.
Agricola
Right arrow Articles by Fu, Y.-B.
Related Collections
Right arrow Other Oil Crops
Right arrow Plant Genetic Resources
Right arrow Crop Genetics

PLANT GENETIC RESOURCES

Geographic Patterns of RAPD Variation in Cultivated Flax

Yong-Bi Fu*

Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, Canada S7N 0X2

* Corresponding author (fuy{at}agr.gc.ca)


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Geographic studies of plant molecular diversity can provide insights into plant domestication and enhance plant germplasm management and utilization, but such studies are lacking in cultivated flax (Linum usitatissimum L.). The objective of this study was to assess the geographic patterns of flax variability in a world collection of cultivated flax by random amplified polymorphic DNA (RAPD) markers. Sixteen RAPD primers were applied to screen 2727 flax accessions representing 63 countries and one group of unknown origin, and 149 RAPD bands were scored for each accession. Analyses of the RAPD data revealed a wide range of occurrence frequencies of polymorphic bands from 0.0004 to 0.9978 with an average of 0.537. The majority (84.2%) of the RAPD variation resided within accessions of each country and only 15.8% of the variation was present among accessions of different countries. Grouping the accessions into 12 major regions explained 8.2% of the RAPD variation. Accessions from the East Asia and European regions were most diverse, but accessions from the regions of Indian Subcontinent and Africa were most distinct. Accessions from the West Asia region were genetically more related to those from the Africa region and less to those from the Indian Subcontinent region. These findings are significant for understanding flax domestication and also are useful in classifying intraspecific diversity of cultivated flax, establishing a core subset of the flax collection, and exploring new sources of genes for flax improvement.

Abbreviations: AMOVA, analysis of molecular variance • PGRC, Plant Gene Resources of Canada • RAPD, random amplified polymorphic DNA • UBC, University of British Columbia


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 
P LANT GENE RESOURCES of Canada (PGRC; the Canadian national seed genebank) at Saskatoon maintains a flax collection consisting of 2813 active accessions of cultivated flax from 69 countries and 54 accessions of 26 wild species in the genus Linum (Diederichsen et al., 2002). To support the management and utilization of flax germplasm, the flax collection was characterized from 1999 to 2001 by the RAPD technique (Williams et al., 1990). The characterization generated DNA fingerprints for each accession at 149 RAPD loci screened from 16 informative RAPD primers. These DNA fingerprints represent the first comprehensive molecular dataset ever obtained for a genebank collection of such size and, thus, provide an opportunity to address not only the issues associated with the management and utilization of flax germplasm (Fu et al., 2002a) but also the domestication of cultivated flax (Fu et al., 2002c).

Flax has been cultivated for oil and fiber for several thousand years, but its domestication remains poorly understood (Zohary and Hopf, 2000). Evidence from archaeological (Helbaek, 1959; Zohary and Hopf, 2000), genetic (Tammes, 1923; Gill and Yermanos, 1967; Fu et al., 2002c), and phenotypic (Diederichsen and Hammer, 1995) studies suggests that the pale flax (L. angustifolium Huds.) is the most likely progenitor of cultivated flax and the likely place of origin was within the Near East region (Zohary and Hopf, 2000). However, the Near East does not fully match the dominant regions of great flax diversity: Indian Subcontinent, Abyssinian, Near East, and Mediterranean (Vavilov 1926; 1951). The flax types from the Indian Subcontinent and Abyssinian regions were found to be morphologically more diverse than those from the Near East and Mediterranean regions (Vavilov, 1926; 1951). It is possible that Vavilov's regions of flax diversity reflected only the multiple domestications which occurred at these regions over time, not necessarily the true place of flax origin, as the origin of a crop plant is diffuse in both time and space (Harlan 1956; 1971). If this reasoning is correct, analyses of geographical patterns of flax variation should yield more information on the number of flax domestication centers and how these centers are genetically connected (Harlan, 1975; Sokal et al., 1991) and less on the region of flax origin (Harlan, 1986). However, such geographic analyses have received little attention (Vavilov, 1926; Elladi, 1940; Dillman, 1953; Chandra and Makhija, 1979), particularly through the application of molecular markers.

Exploitation of flax genetic resources for flax improvement requires knowledge of the range and structure of the genetic variability present in flax gene pools, but comprehensive characterizations of the existing flax germplasm held in the world collections are lacking, particularly using molecular techniques. Early characterization efforts based on agrobotanical characters revealed four major regions of flax diversity as mentioned above (Vavilov, 1926; 1951) and several unique groups of cultivated flax such as fiber flax, intermediate flax, large-seeded flax, and dehiscent flax (Dillman, 1953; Kulpa and Danert, 1962). Recent RAPD analyses showed (i) that RAPD variation in flax was generally low; (ii) that more variation existed in landraces than cultivars; (iii) that more variation was detected in linseed, than fiber, flax; (iv) that North American linseed cultivars had more variation than those from the other countries; (v) that more variation was found in the Canadian linseed cultivars released before 1980 than those released after 1980; and (vi) that the genetic erosion in the century-long North American linseed breeding programs was not significant (Fu et al., 2002b; 2003b). While these variation patterns are significant for the improvement of North American flax, they are limited in helping us understand how large the genetic variation in a flax collection is, how the flax gene pool is structured, how a core set of flax germplasm should be sampled, and how the flax germplasm could be effectively managed. To address these issues requires a molecular screening of the world collection of cultivated flax and a pattern analysis of germplasm variability (Fu et al., 2002a).

The objective of this analysis was to assess the geographic patterns of RAPD variation in the world collection of cultivated flax maintained at PGRC. Molecular markers are less affected by environmental factors than those morphological and botanical characters previously used to describe flax variation and should be more informative in determining geographic patterns of flax diversity (Harlan, 1986).


   MATERIALS AND METHODS
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Plant Materials
The PGRC collection of flax germplasm is largely a duplication of the flax collection maintained in the United States National Plant Germplasm System. Newly acquired germplasm include 94 accessions developed in Canada and 571 accessions of diverse origin from Russia, Germany, and the Czech Republic. During rejuvenation from 1999 to 2001, 238 additional accessions with defined phenotypic differences were newly selected from mixed samples. Currently, the PGRC flax collection consists of 2813 active accessions of cultivated flax and stands as the seventh largest collection of flax genetic resources in the world (Diederichsen et al., 2002).

A total of 2727 accessions of cultivated flax (Table 1) were subjected to RAPD characterization from 1999 to 2001. These accessions represented 63 countries with some of uncertain origin as a control. To assess the region(s) with the greatest diversity, accessions of known country origin were grouped according to the region classification of Zhukovsky (1968) [and later described by Zeven and Zhukovsky (1975)] for 12 crop diversity centers, not the eight "centers of origin" of Vavilov (1926)(1951). Zhukovsky's classification was an expansion of Vavilov's centers from eight to 12 regions by taking into account the evidence newly accumulated and thus is more informative (Zeven and Zhukovsky, 1975). On the basis of Zhukovsky's classification, no accessions represented the second region (Indochina–Indonesia), while there were 909 accessions originating from the ninth region (Europe–Siberia). To reduce the impact of accession sizes in this analysis, the ninth region was separated to two subregions: East Europe (9a) and West Europe (9b).


View this table:
[in this window]
[in a new window]
  		Table 1. Random amplified polymorphic DNA (RAPD) variations for 2727 flax accessions representing 63 countries, 12 proposed regions, and one group of unknown origin.

 
DNA Extraction and RAPD Analysis
About 10 to 15 seeds of each accession were sampled from the PGRC flax collection and grown in a greenhouse at the Saskatoon Research Centre, Agriculture and Agri-Food Canada. Young leaves were collected from 10 3- to 4-wk-old seedlings, bulked for each accession, freeze-dried for 3 to 5 d, and stored at –80°C. DNA was extracted from 50 mg of dry tissue in a 2-mL Eppendorf tube by shaking with three glass beads, adding 1 mL of 95°C extraction buffer [0.1 M Tris-HCl (pH 8.0), 10 mM EDTA, 1 M KCl], and incubating at 80°C for 10 min with occasional agitation. The homogenate was centrifuged to remove cell debris. The supernatant was treated with 5 µL of 10 g L–1 RNase A and DNA was precipitated with isopropanol. The DNA was suspended in 50 µL of water and then quantified by fluorimetry using Hoechst 33258 stain (Sigma Chemical Co., St. Louis, MO). Typical yields were 10 to 15 µg of DNA and all of the DNA samples were diluted to 2.5 ng µL–1.

Each polymerase chain reaction (PCR) contained 10 ng of DNA as template, 4 U of Taq DNA polymerase (BRL, Mississauga, ON), 50 mM KCl, 2.5 mM MgCl2, 200 µM of each dNTP, and 0.2 µM decamer primer [University of British Columbia (UBC), Vancouver, BC]. All PCR reactions were performed in the PTC-200 DNA Engine thermocycler (MJ Research, Watertown, MA) with 1.5 min at 95°C, followed by 35 cycles of 20 s at 95°C, 1 min at 36°C (ramp 1°C/s), 1.5 min at 72°C, and a final 7 min cycle at 72°C. PCR products were separated in 2% (w/v) agarose gels in 1x TAE by electrophoresis at 100 V for 3 h. Gels were stained in ethidium bromide and photographed on a digital gel-documentation system (Stratagene, La Jolla, CA).

RAPD analyses were performed with 16 informative UBC RAPD primers (Table 2). These primers were selected from previous primer screenings and various tests of reproducibility as described in Fu et al. (2002b)(2003a). The analyses generated about 1800 gel images, from which a total of 149 polymorphic bands were scored independently by two individuals as present (1), absent (0), or uncertain (9) for each accession.


View this table:
[in this window]
[in a new window]
  		Table 2. Variations of random amplified polymorphic DNA (RAPD) markers observed for 16 RAPD primers synthesized by University of British Columbia (UBC).

 
Data Analysis
The scored bands were analyzed for the level of polymorphism with respect to each primer, the accession country of origin, and the proposed accession region by counting the number of polymorphic bands and generating the summary statistics on the band frequencies. To visualize the variation pattern, the numbers of polymorphic bands were plotted against their frequencies of occurrence in all assayed accessions. To assess the impact of accession size on the polymorphism observed for each country and region, a regression was made by SAS PROC REG (SAS Institute, Inc., 2004) on the number of accessions over the number of polymorphic bands, the mean band frequency, and within-country (or within-region) variation measured from the sum of squares from the below analysis of molecular variance (AMOVA; Excoffier et al., 1992).

To assess RAPD variations across the countries and regions, an AMOVA was performed by Arlequin version 2.001 (Schneider et al., 2002). This analysis enables partitioning of the total RAPD variation into within- and among-country (or region) variation components, and provides a measure of inter-country (or inter-region) genetic distances as the proportion of the total RAPD variation residing between flax accessions of any two countries (or regions) (called Phi statistic; Excoffier et al., 1992). Models involving one level of structuring (country) and two levels of structuring (region and country) were applied. Significance of resulting variance components and inter-country (or inter-region) genetic distances was tested with 10100 random permutations. To assess the genetic relationships of the flax accessions of different countries and regions, the inter-country (and inter-region) distance matrices of the Phi statistic were analyzed by NTSYS-pc 2.01 (Rohlf, 1997) and clustered with the algorithm of unweighted pair–group methods using arithmetic averages.


   RESULTS AND DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
CONCLUSIONS
 REFERENCES
 
RAPD Variation
A total of 149 polymorphic RAPD bands screened from 16 informative RAPD primers were analyzed in this study. The number of polymorphic bands scored for any specific primer ranged from four (for the primer UBC 790) to 16 (for the primer UBC 337) with an average of nine (Table 2). These bands were presumably detected randomly from the 2n (=30) flax chromosomes and would have covered the whole genome with an average genetic distance up to 20 cM between any two bands. However, the true genome coverage by these RAPD loci requires further investigation.

The occurrence frequencies of the 149 polymorphic bands ranged from 0.0004 to 0.9978 with an average of 0.537. Most of the polymorphic bands appear to be either frequently or infrequently present in all accessions (Fig. 1). Eighteen polymorphic bands (12%) were present in most of the accessions (with frequency of 0.95 or greater) and 42 polymorphic bands (28%) present only in a small proportion of accessions (with frequency of 0.15 or lower). Such a pattern of variation is expected for a plant germplasm collection with many accessions representing breeding lines or cultivars, as artificial selection within inbreeding species generally forces both dominant and recessive alleles toward fixation (Villand et al., 1998). For polymorphic bands detected by specific primers, the average band frequency varied greatly from 0.341 for the primer UBC403 to 0.709 for the primer UBC465 (Table 2), indicating the heterogeneity of the detected flax genomes. Thus, a large amount of genetic diversity exists in the world collection of cultivated flax.



View larger version (26K):
[in this window]
[in a new window]
  		Fig. 1. Number of polymorphic markers of random amplified polymorphic DNA (RAPD) in relation to their frequencies of occurrence in all flax accessions.

 
Variation among Accessions of Different Countries
The number of accessions for each country varied greatly (Table 1). There were 16 countries with only one accession and 47 countries with two to 539 accessions. The country with the most accessions was the USA (with 539 accessions), followed by Russia (309), India (286), Argentina (168), France (157), Hungary (136), Turkey (125), Canada (113), and Pakistan (103). This distribution of accession sizes across countries of origin was significantly associated (r = 0.6; P < 0.0001) with the variation in the number of polymorphic bands observed for each country. Thus, a country with more accessions displayed more polymorphic bands. However, associations of accession size were not observed with the mean band frequency and the within-country variation calculated from the sum of squares from AMOVA. The mean band frequency varied among the 47 countries and ranged from 0.46 (UK) to 0.61 (Iraq and Kazakhstan). The within-country variation also varied among the 47 countries and ranged from 12.0 (Azerbaijan) to 32.2 (UK) (Table 1). The countries with the most within-country variations were UK (32.2), Italy (30.2), Romania (29.4), Hungary (27.4), Australia (27.2), and France (27.2). On the basis of AMOVA with one level of structuring, the among-country variation was significant, and it accounted for 15.8% of the total RAPD variation in the flax accessions assayed (Table 3). Thus, the majority (84.2%) of the RAPD variation still resided within the accessions of a country.


View this table:
[in this window]
[in a new window]
  		Table 3. Results for analysis of molecular variance (AMOVA) for 2727 flax accessions representing 63 countries, 12 proposed regions, and one group of unknown origin based on 149 random amplified polymorphic DNA (RAPD) markers and two models of structuring.

 
Assessments on the genetic relationships of flax accessions of 47 countries with more than one accession revealed two major distinguishable groups (Fig. 2). The first group included accessions from India and Pakistan and the other consisted of accessions from Iran, Syria, Turkey, and Iraq. These two groups largely corresponded with their geographic regions, but accessions from the other countries were clustered in various subgroups with less correspondence with geographic origins. For example, accessions from Algeria and Morocco are genetically similar to those from Australia, Greece, and Hungary. Accessions from Canada and USA were genetically similar to those from New Zealand and Kenya. These results appear to indicate the genetic differentiation among the flax accessions of various countries were relatively weak, except for those of the first two distinguishable groups.



View larger version (22K):
[in this window]
[in a new window]
  		Fig. 2. Genetic relationships of 2711 flax accessions representing 47 countries and one group of unknown origin in a dendrogram based on their differences reflected in random amplified polymorphic DNA (RAPD) markers. The region code is given in the parentheses following the country label.

 
Variation among Accessions of Proposed Regions
The flax accessions assayed represented only 11 out of 12 regions originally proposed by Zhukovsky (1968) for crop diversity centers (Table 1). Also, the Central America region was underrepresented with only three accessions (Table 4). The region with the most number of accessions was North America (i.e., having 652 accessions), followed by East Europe (638 accession), Indian Subcontinent (389), and West Europe (271). However, this distribution of accessions for the proposed regions was not significantly associated with the variation in the number of polymorphic bands, the mean band frequency, and the within-country variation measured from the sum of squares from AMOVA. The number of polymorphic bands for each region ranged from 35 (Central America) to 141 (North America). There was a narrow mean band frequency at the region level, ranging from 0.506 (Africa) to 0.579 (East Asia). East Asia displayed the most within-region variation (28.6), followed by East Europe (27.6). Excluding the underrepresented region of Central America, accessions from the Indian Subcontinent region had the lowest within-region variation (21.7). This indicates accessions within the Indian Subcontinent region were genetically more similar than those within the other regions.


View this table:
[in this window]
[in a new window]
  		Table 4. Comparison of random amplified polymorphic DNA (RAPD) variations for flax accessions of different regions.

 
On the basis of AMOVA with two levels of structuring, the among-region variation was significant, and it accounted for 8.2% of the total RAPD variation (Table 3). Thus, RAPD variation exists among accessions of different regions but is relatively small. Assessments on the RAPD variations of among-region accessions revealed accessions from the Indian Subcontinent region were the most genetically distinct from those of the other 10 proposed regions, followed by the accessions from Africa, West Asia, and Central Asia (Table 5). Accessions from the Indian Subcontinent region displayed the largest genetic distances with accessions of the other regions (ranging from 0.19–0.32), followed by those of Africa (0.14–0.27), West Asia (0.06–0.22), and Central Asia (0.05–0.19). The genetic distances obtained among the other seven regions largely were smaller than 0.10. Assessing the genetic relationships of the flax accessions originating from different regions revealed four clear, distinct clusters representing the Indian Subcontinent, Africa, West Asia–Central Asia, and the other eight proposed regions including Mediterranean. Accessions from the Indian Subcontinent region were genetically less related to those from the other regions, followed by those from Africa, West Asia–Central Asia, and the remaining regions. Further assessment on these regional patterns with respect to the original four centers of flax diversity proposed by Vavilov (1926)(1951) confirmed N.I. Vavilov's observations on flax diversity as reflected by morphological and botanic trait evaluation.


View this table:
[in this window]
[in a new window]
  		Table 5. Genetic distances among flax accessions of different regions measured by the proportion of the total random amplified polymorphic DNA (RAPD) variation residing between regions ({Phi}st; below the diagonal). Significance of each {Phi}st value is given above the diagonal as the probability obtained by 10100 random permutations that a random {Phi}st value was greater than observed value.

 
Variation Patterns and Centers of Flax Diversity
The RAPD analysis presented here represented the first attempt to assess the regional patterns of molecular variation in a crop species from such a large scale of plant germplasm. The variation patterns revealed here (Fig. 2 and 3) probably reflect all the possible "centers of diversity" (or distributions of flax variability) presently existing in the world gene pool of cultivated flax. These should include those, if any, newly developed by extensive exchanges of flax germplasm that have taken place worldwide over the last 60 yr (Peeters, 1988). However, a comparison of these regional patterns of flax variation with those traditional "centers of diversity" formulated in 1920s by N.I. Vavilov (1926)(1951) revealed little discrepancy. This may reflect the fact that the exchanged germplasm was rarely utilized in flax breeding programs and still maintained most of the initial variability captured from originating countries.



View larger version (20K):
[in this window]
[in a new window]
  		Fig. 3. Genetic relationships of 2727 flax accessions representing 12 proposed regions and one group of unknown origin in a dendrogram based on their differences reflected in random amplified polymorphic DNA (RAPD) markers. Australia–NewZ stands for the Australia–New Zealand region; Indian Subcont. for the Indian Subcontinent region.

 
It is difficult to interpret these regional patterns with respect to the place of flax origin (Zeven and Zhukovsky, 1975; Durrant, 1976), but these findings appear to offer some insight to flax domestication, if one reasons with the diffuse theory of Harlan (1986). Possibly, these regional patterns of flax variability reflect the multiple centers of flax cultivation (Harlan, 1971), since there are significant differences in diversity across regions. Also, some centers of domestication are genetically more related than the others (Fig. 3) and might reflect the geographical direction of flax cultivation. For example, African flax shows a greater relatedness to those from the West Asia and Central Asia regions but a lower relatedness to those from the Indian Subcontinent region. This implies that African flax may have been spread in domestication from the Near East region, and not the Indian Subcontinent region. If this reasoning is correct, connecting these centers of domestication in chronological order will enhance our understanding of flax domestication. Testing this reasoning could proceed with DNA sequence analyses of flax from various regions (Jaenicke-Despres et al., 2003), which allows for molecular dating of domestication events. However, archaeobotanical support from these regions is definitely needed, particularly for the Indian Subcontinent and Africa regions where flax fossils were largely lacking (Zohary and Hopf, 2000).

Implications for Germplasm Management and Utilization
These regional patterns of RAPD variation are significant for the management and utilization of flax germplasm, particularly for those in the PGRC collection. The PGRC collection, although well representative for some countries and regions, could be further enhanced if specific field collections of flax germplasm are performed in the regions of large variation such as East Asia and Europe and of great distinctness such as Indian Subcontinent and Africa. Incorporating these regional patterns of variation in evaluations of intraspecific classification of flax diversity such as those of Dillman (1953) and Kulpa and Danert (1962) would make the grouping of intraspecific diversity more informative for germplasm management (Diederichsen and Fu, 2004). Exploring these variation patterns with respect to environmental factors or connecting these patterns to the resistance patterns of flax diseases may result in grouping of accessions with similar adaptedness or disease resistance and thus would facilitate the search for unique genotypes from the collection.

Establishing a core subset of flax accessions from the PGRC collection can enhance the management and utilization of flax germplasm (Brown and Spillane, 1999), but challenges remain in the applications of sampling to capture the existing variability. However, the geographic patterns of flax variability revealed here provide some baseline information for assigning the weighting each country or region could contribute to a core subset and for developing effective sampling strategies. Accessions from the Indian Subcontinent, Africa, and West Asia regions may be weighted more than those from other regions. Large weighting could also be given to the accessions from the regions and countries of great diversity (Tables 1 and 4). To capture more variation in a core subset, a random sampling should be stratified at the country level, as more variation resided among countries than among proposed regions. Generally, a random stratified sampling with size allocation proportion to the level of variation existing in a country should be considered (Brown, 1989), but such sampling may not necessarily be optimal. This issue can be addressed, given the entire collection was characterized. Efforts are being made to assess the effectiveness of different sampling methods in capturing genetic variation in a core subset, and eventually a well-representative core subset will be developed for the PGRC collection.

Our analysis revealed that a large amount of genetic diversity existed in the flax collection. About 84% of the variation detected resided within the accessions of a country and 16% among accessions of different countries. Thus, selection of breeding materials could be made from the flax germplasm within a country for flax improvement. Also, about 8% of the RAPD variation resided among accessions of different regions (Table 3), and selection of breeding materials from germplasm of different regions is also possible. Exploring new source of genes within the existing gene pool of cultivated flax might be made initially toward accessions originating from the Indian Subcontinent and Africa regions, where the most distinct germplasm exists. To broaden the genetic base of breeding populations, inclusion of some flax germplasm originating from the East Asia and East Europe regions should be considered, at least for initial screening. Developing specific core subsets of flax accessions on the basis of these variation patterns to facilitate the initial screening of genes of particular interest is also feasible. These core subsets could save some effort in the exploration of new breeding germplasm for flax improvement.


   CONCLUSIONS
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
REFERENCES
 
The RAPD analysis reported here represented the first attempt to assess the regional patterns of molecular genetic variation in a crop species from such a large scale of plant germplasm. The PGRC flax collection displayed a large amount of genetic diversity in flax germplasm. The majority (84.2%) of the RAPD variation resided within accessions of each country and only 15.8% of the variation was present among accessions of different countries. Grouping the accessions into 12 major regions explained 8.2% of the RAPD variation. Accessions from the East Asia and European regions were most diverse, but accessions from the regions of Indian Subcontinent and Africa were most distinct. Accessions from the West Asia region were genetically more related to those from the Africa region and less to those from the Indian Subcontinent region. These findings provide additional insights to flax domestication and also are useful in classifying intraspecific diversity of cultivated flax, establishing a core subset of the flax collection, and exploring new sources of genes for flax improvement.


   ACKNOWLEDGMENTS
 
The author thanks Gregory Peterson for his technical assistance on the RAPD characterization of the flax germplasm; Dallas Kessler, Dave Williams, and Peter Kusters for their assistance in sampling seeds and planting them in the greenhouse; and Rong-Cai Yang and Axel Diederichsen for their helpful comments on the manuscript.

Received for publication June 4, 2004.


   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 


Related articles in Crop Science:

THIS ISSUE IN CROP SCIENCE

Crop Science 2005 45: xiii. [Full Text]  



This article has been cited by other articles:


Home page
 Crop Sci.Home page
A. J. Jhala, L. M. Hall, and J. C. Hall
Potential Hybridization of Flax with Weedy and Wild Relatives: An Avenue for Movement of Engineered Genes?
Crop Sci., May 1, 2008; 48(3): 825 - 840.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Figures Only
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Related articles in Crop Science
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fu, Y.-B.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Fu, Y.-B.
Agricola
Right arrow Articles by Fu, Y.-B.
Related Collections
Right arrow Other Oil Crops
Right arrow Plant Genetic Resources
Right arrow Crop Genetics

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality		 The Plant Genome