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Fig. 4. BamHI digestion of PCR products obtained from genomic DNAs amplified using CAPS primers S30-13L and AGA7-759R. Genomic DNAs were extracted from Neepawa (Nee) and Neepawa*6/Triticum speltoides F-7-3 (NeeF7), and from five BC6F2 plants. Letters "BS" and "SS" indicates plants heterozygous and homozygous for the presence of Lr51, respectively. Parental line Neepawa (Lane 7) is the only BB plant in this figure. The black arrowhead indicates the 819-bp PCR amplification product from the S genome allele and the gray arrowhead indicates the larger of the two BamHI digested fragments from the B genome allele of XAga7. "M" indicates the size molecular marker (100-bp ladder, Biodynamics).
