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Fig. 4. Leaf appearance rate (leaves GDD–1) in Italian ryegrass following single or double 15-h exposure to minimum temperatures of –2.5 and –5.0°C, a single exposure to –7.5°C or in a control treatment maintained at the growth chamber temperature regime with minimum night temperature of 0°C. EXP = single 15-h exposure in uninsulated Cone-tainer, EXP2 = two 15-h exposures in uninsulated Cone-tainer, INS = single 15-h exposure in insulated Cone-tainer, INS2 = two 15-h exposures in insulated Cone-tainers. Vertical bar shows LSD = 0.0033 (P = 0.05) for comparison of means.