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Fig. 4. The suppression of P34 by sense cosuppression is shown in the adjacent blots. The three panels show replicate samples stained for total protein with amido black, and labeled with either anti-P34 monoclonal antibody or with IgEs from a soybean-sensitive person. Lane 1 is a transgenic control of another soybean suppressing the 
/' conglycinin (arrowhead) that does contain P34 (Kinney et al., 2001). Lane 2 is a transgenic soybean line containing a construct that includes the P34 cDNA that did not induce suppression. Lane 3 is a T3 generation homozygous transgenic soybean with the P34 construct inducing the complete suppression of P34 accumulation. Note the complete absence of P34 assayed by the monoclonal antibody and IgE cross-reactivity using sera from a soybean-sensitive person. Reprinted from Plant Physiol. 132: 36-43 (2003).
