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USDA-ARS Sugarcane Field Stn., 12990 US Hwy 441 N., Canal Point, FL 33438
* Corresponding author (sedme{at}saa.ars.usda.gov).
| ABSTRACT |
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Abbreviations: CP, Canal Point CY, cane yield EAA, Everglades Agricultural Area SC, sucrose content SY, sugar yield
| INTRODUCTION |
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Successful maintenance of sugarcane within the portfolio of crops grown in the EAA has been made possible by two structured breeding programs (USDA, Canal Point and USSC, Clewiston) that share the same objective, namely the development of new cultivars with high and stable yields for South Florida. Improvements in cultural practices and milling efficiency have also contributed to the establishment of sugarcane as a viable and economic crop in the EAA.
Concerns exist that sugarcane yield may soon plateau or has already reached a plateau caused by exhaustion of genetic diversity (McCloud, 1977; Mariotti, 2002). Most of the sugarcane cultivars in the world can be traced back to a very limited number of parents used to synthesize the original interspecific hybrids bred in Indonesia and India (Berding and Roach, 1987; Deren, 1995). For most of the 20th century, sugarcane breeding worldwide thrived essentially on crossing progeny within this original population. The CP program tended to exploit a more diverse gene pool to transfer genes that conferred high yield and resistance to both biotic and abiotic stresses into the cultivated background (Tai and Miller, 1978). The CP breeding program has also adopted a "shuttle" breeding strategy (Young and Frey, 1994) that alternates clonal selection between optimum (organic soils in the initial three stages) and multilocation conditions (both organic and sandy soils in the final two stages) at representative farm sites. This philosophy also capitalizes on reentry of the best performing clones into the crossing program. The CP cultivars are currently grown on roughly 90% of the Florida sugarcane hectarage (Glaz et al., 2003) and are used extensively in many parts of the world, particularly the Caribbean and Central America (J.D. Miller, personal communication).
No attempt has been made to assess the impact of breeding on the improvement of sugarcane for Florida. Baver (1963) and Hogarth (1976) attributed 50 and 75% of the gains in sugarcane yields to genetics for Hawaii and Australia, respectively. Similar assessments have been made in other crops (Wych and Rasmusson, 1983; Duvick, 1992a; Lauer et al., 2001) to evaluate the genetic contribution to overall progress and to shed light on future strategies needed for advancement. Genetic improvements have contributed to about 50% of the yield gains attained in major U.S. crops (Fehr, 1984; Duvick, 1992b; Frisvold et al., 1999). Progress has been assessed with traditional yield trials or with long-term yield trials, by comparing performance of both old and new cultivars in common environments under current management strategies (Wych and Rasmusson, 1983). Both of these methods have an upward bias resulting from the difficulty of not being able to remove the effects of cultural practices (Langer et al., 1978). However, yield trials across time, which include long-term reference cultivars, can enhance the reliability of the comparison (Langer et al., 1978; Peltonen-Sainio and Karjalainen, 1991). Other researchers have relied on historical records of yields from commercial production or cultivar trials conducted at several locations across several decades to estimate genetic gains and contribution to yield improvements (Langer et al., 1978; Peltonen-Sainio and Karjalainen, 1991; Specht et al., 1999). This study on sugarcane also used historical data from both commercial production and the last stage of experimental selection trials, which included long-term reference cultivars, to assess the genetic contribution to yield gains in the Florida sugarcane industry.
Quantifying genetic gains in sugarcane is an important step in the renewed efforts by the Florida sugar industry to maximize economic returns while protecting the environment and to improve end-product marketing (Shine, 2002). The objectives of this study were to estimate the overall rate of cultivar improvement achieved by the Florida sugarcane industry, to assess progress made for each of three crop ages (plant cane and first and second ratoons) grown on both organic and sandy soils, and to separate the genetic contribution from that of cultural practices.
| MATERIALS AND METHODS |
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Three major yield variables were measured: sucrose content (SC; kg Mg1 of cane), cane yield (CY; Mg ha1), and sugar yield (SY; Mg ha1), by methods described by Arceneaux (1935) and Legendre (1992). Before 1993, whole plots were weighed in the field with a tractor-mounted device for calculation of cane and sugar yields (Glaz et al., 1993). Since 1993 and starting with the CP 89 series, cane and sugar yields were estimated from 10-stalk sample weights and stalk numbers, necessitated by labor shortage after the sugarcane industry shifted to mechanical harvesting (Glaz et al., 1994). To avoid any bias, mean yields of cultivars released before or after 1993 were regressed separately on years of release to calculate rates of increase due to genetic improvement.
The GLM procedure in SAS v. 8.2 (Littell et al., 2002) was used to regress performance data for SC, CY, and SY on years of production or of release as independent variables. Years were coded as 1 (1968) to 33 (2000) before carrying out single-degree-of-freedom regression analyses to determine the rate of genetic improvement in sugarcane across the 33-yr period. Commercial production data were regressed on years to obtain mean yield increase across time as realized gain and trends were also observed for each of the following three periods: 1968 to 1980 (Period 1), 1981 to 1990 (Period 2), and 1991 to 2000 (Period 3). Least-square means across clones, sites, and crop ages were used to assess the quality of the clones reaching selection Stage IV. Least-square means of released cultivars were also calculated to determine maximum genetic gain across time. The slopes or b values, significant at the 0.05 probability level, obtained from linear regression analyses, were considered as gains.
Long-term reference cultivars were used to calculate the relative contribution attributed to plant breeding. Commercial sugar yields for the Florida industry were averaged for each of the three periods, corresponding to the time when a released cultivar dominated, and compared with mean yields of these cultivars obtained in Stage IV trials for the same time periods, according to Wych and Rasmusson (1983). Reference cultivars were CP 63-0588 for Period 1; CP 70-1133, CP 72-1210, CP 72-2086, CP 73-1547, and CP 80-1827 for Period 2; and CP 78-1628, CP 80-1743, CP 88-1762, and CP 89-2143 for Period 3. Percent increases within periods, obtained from Stage IV trials, were averaged and divided by increases realized in commercial fields to obtain an estimate of the overall contribution attributable to the Canal Point sugarcane improvement program, in accordance with Wych and Rasmusson (1983).
Annual values of the additional producer benefits were estimated from mean commercial sugar yields for the 1968 to 2000 period and converted to year 2000 dollar values, based on the annual hectarage harvested for sugar, the portion of the rate of progress for sugar yield attributable to breeding, and a base price of $440.00 Mg1 of raw sugar. This was accomplished by compounding past dollar amounts to the year 2000, using a compound interest formula, with interest rates of 0% and 5% (Frisvold et al., 1999; Yu et al., 2002). Cumulative gross benefits were calculated after summing present values across the 33-yr period considered. The present value (PV) in year 2000 dollars was calculated with the following formula:
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| RESULTS AND DISCUSSION |
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From 1968 to 2000, SY improved significantly (P < 0.05) at linear rates of 0.10 and 0.16 Mg ha1 yr1 under commercial fields and Stage IV trials, respectively (Fig. 1c). This corresponded to respective gains of about 47 and 53% across 33 yr. Sugar yield did not change significantly (P > 0.05) during Period 1, but it improved at rates of 0.17 and 0.18 Mg ha1 yr1 during Period 2 and Period 3, respectively, under commercial field production (Table 1). In Stage IV trials, SY improved at a faster linear rate during Period 3 (0.62 Mg ha1 yr1) than during Period 2 (0.13 Mg ha1 yr1). SY is the product of CY and SC. Since there was no increase in CY or SC during Period 1, no accompanying gains were observed in SY, as expected. Gains in SY during Period 2 resulted from an improvement in SC, whereas during Period 3, improvements in both CY and SC contributed to increases in SY. Many sugarcane breeding programs in the world have relied on improving cane yield to increase sugar yield per hectare (Mariotti, 2002). However, there is an added incentive to increase sugar yield by improving sucrose content, because milling efficiency tends to decline with high sugarcane biomass and some sugarcane payment systems impose a penalty for high-tonnage low-sucrose cultivars (Legendre, 1992).
The 1968-1980 period was dominated by cultivars such as CL 41-0223 and CP 63-0588. This period was characterized by steady expansions of the industry onto cold-prone fields away from Lake Okeechobee and also by efforts to increase sugarcane production on shallower organic soils and on sandy soils (James, 1970; Aleman, 1984). A two-fold increase in planted hectarage (6140 ha yr1) was recorded during Period 1 from 75000 ha in 1968, as compared with 3868 and with 3640 ha yr1 during Period 2 and Period 3, respectively (data not shown). Moreover, during Period 1, the primary selection emphasis was on CY and better adapted cultivars, with a minimum standard imposed on SC. This period also coincided with the appearance of new diseases and an increased incidence of smut [caused by Sporisorium scitaminea (formerly Ustilago scitaminea H. Syd. and P. Syd.)], leaf scald (caused by Xanthomonas albilineans Asby), and rust (caused by Puccinia melanocephala H. Syd. and P. Syd.). Under these disease pressures, Florida sugarcane breeders adjusted their strategies in response to industry needs, which likely contributed to absence or nonevidence of yield gains during Period 1. As selection pressure was applied on both SC and CY, all three traits improved simultaneously as reflected by the release of high sucrose and/or tonnage cultivars (CP 70-1133, CP 72-1210, CP 72-2086, and CP 80-1827) and better sand-adapted cultivars (CP 70-1133 and CP 73-1547) during the 1981-to-1990 period. Contrary to belief, gains in SY may be more associated with gains in SC than with gains in CY as Fig. 1 seems to indicate that new cultivars may allocate assimilates more efficiently toward accumulation of sucrose. A good sampling and pyramiding of the desirable genes (for disease resistance, cold tolerance, and yield) available in the working germplasm might have also contributed to the yield changes observed under both commercial fields and the last stage of selection.
Gains across Soil Types and Crop Ages
There were substantial differences in progress on organic soils versus that on sandy soils (Table 2). Florida sugarcane breeders improved SC (P < 0.01) on organic soils at a rate of 0.81 kg Mg1 of cane yr1. This overall gain was sustained by performance improvements in plant-cane (0.79 kg Mg1 of cane yr1), first-ratoon (0.82 kg Mg1 of cane yr1), and second-ratoon (0.79 kg Mg1 of cane yr1) crops. Improvements for SC were not recorded on sandy soils, regardless of crop age.
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Significant gains in SY for plant-cane (0.25 Mg ha1 yr1), first-ratoon (0.26 Mg ha1 yr1), and second-ratoon (0.12 Mg ha1 yr1) crops grown on organic soils contributed to a significant (P < 0.01) overall increase in SY of 0.21 Mg ha1 yr1 (Table 2). Overall, SY on sandy soils did not change because of the absence of gains for plant-cane and first-ratoon crops. There was, however, a gain of 0.12 Mg ha1 yr1 for the second-ratoon crop.
There were significant improvements in performance in all three crop ages for SC, CY, and SY across soil types (Table 3), contributed mostly by improvements on organic soils (Table 2). Ratooning ability is an important selection criterion in sugarcane breeding programs. Overall gains achieved with the release of improved sugarcane cultivars in the 33-yr period largely reflected favorable progress on organic soils. Greater stress tolerance will be necessary to minimize the difference between gains in performance on organic and sandy soils. Most of the improved cultivars were better adapted to the organic soils of the EAA than to the sandy soils. High-yielding cultivars (CP 70-1133, CP 75-1547, CP 78-1628, and CP 84-1591), adapted to sandy soils, have been released also to the industry across the years and sucrose content of these cultivars grown on sandy soils still ranged from 100 to 150 kg Mg1 of cane. Even though the breeding strategy, espoused by the cooperative Canal Point sugarcane breeding program, is able to advance clones with potential release for sandy soils to Stage IV, the frequency is not as high as that obtained on organic soils. To upgrade and increase the frequency of advanced materials for sandy soils would require specific crosses to be made for the sand-soil environment and to begin testing clones in the early stages directly on sandy soils. However, plant breeding programs usually have to make compromises and establish priorities because of availability of limited resources to breed for the different target environments (Brown and Glaz, 2001).
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Some scientists believe that gains in sugarcane for Florida could be higher on commercial fields, were it not for successive plantings (sugarcanesugarcane rotation system without fallow) and certain restrictions for environmental protection. A review of annual census reports on Florida sugarcane hectarage generally documents a greater area devoted to successive than to fallow plantings (Glaz and Coale, 1987; Glaz, 1994, 1995; Glaz and Ulloa, 1995). Successive planting is part of the rotation system and is being accounted for in the breeding program in the last stage (Stage IV) of selection. Moreover, efforts are being made to address the sustainability of sugarcane to potential changes in agricultural practices (mechanical harvesting, higher water tables, efficiency in phosphorus uptake, etc.) associated with ongoing Everglades restoration and protection efforts (Shine, 2002).
The cumulative benefits to the Florida economy in Year 2000 dollar values, attributed to sugarcane breeding efforts by the cooperative USDA-ARS Canal Point program, were estimated to range between $99 million (0% interest) and $203 million (5% interest) (Table 4). In an assessment of benefits contributed by the University of Nebraska-Lincoln wheat breeding program (associated with a considerably larger hectarage), Yu et al. (2002) reported benefits that ranged from $200 million (0% interest) to $485 million (5% interest) across a period of 30 yr.
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| CONCLUSIONS |
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The potential for selecting higher yielding clones has increased across the years and helps explain the advances documented in the Florida sugarcane industry. Effective sampling and pyramiding of the desirable genes available within the working germplasm led to the improvements in SC, CY, and SY. With the polyploid nature of sugarcane and keeping in mind that only about 12 generations have passed since inception of sugarcane breeding, more yield progress is to be expected. Continued use of a widened gene pool will still be necessary to maintain future increases in yield potential.
Received for publication December 29, 2003.
| REFERENCES |
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