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Fig. 9. NK603 zygosity test. PCR based assay using three oligonucleotides D, E, and F. The schematic diagram (Panel A, not to scale) shows relative placement of oligonucleotides and the expected sizes of amplification products on non-transgenic (Wt) and NK603 templates. The assay was conducted on genomic DNA from a non-transgenic plant (Panel B, lane 3, B73 inbred), a plant hemizygous for the NK603 insertion (lane 4, B73 BC3 composition), a plant homozygous for the NK603 insertion (lane 5, B73 BC2S2 composition), or plants hemizygous for similar but independent transgenic events (Lane 6, event NK560; Lane 7, event NK561; Lane 8, event NK600). Lane 2 is a template-minus control reaction. Lane 1, 100-bp DNA ladder size standard.
