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Fig. 6. Southern analyses of NK603 transgenic elements. Corn genomic DNA was digested with the indicated restriction enzymes, electrophoresed in replicate 0.8% (w/v) agarose gels, transferred to a nylon membrane, hybridized to 32P-labeled segments of PV-ZMGT32, and subjected to autoradiography. Individual lanes: 1) EcoRV digest of 10 µg B73 inbred; 2) EcoRV digest of 10 µg B73 inbred genomic DNA supplemented with 29 pg of PV-ZMGT32; 3) EcoRV digest of 10 µg NK603 B73 BC5S3 genomic DNA; 4) MscI digest of 10 µg of B73 BC5S3 genomic DNA. Panel A, rice Act1 promoter probe; Panel B, CTP/CP4 EPSPS probe; Panel C, e35S promoter; Panel D, NOS transcriptional termination sequence probe. Molecular weight markers are indicated on the side of each autoradiogram (kb, kilobase pairs).
